以RAPD及SSR分子標誌進行樹薯種原遺傳歧異分析及種原鑑定

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(1)‫؀‬᨜ልᄐઔߒ (J. Taiwan Agric. Res.) 56(4):327-337 (2007). аRAPDϷSSRϩη኱ᇞ຾Չᐋᖘᅿচᒪ໺‫ݔ‬౦ϩ‫݋‬ Ϸᅿচ᠘‫ۓ‬1 ഋॊ2,4 ण‫ے‬৓3 ݅ߪက2 㛧尐 ຫ૪Εૃࡲ୳ΕࣥঊᆠΖ2007Ζ‫ א‬RAPD ֗ SSR ։՗ᑑ፾ၞ۩ᖫ᜸ጟ଺ᙊႚࣴฆ։࣫ ֗ጟ଺ᦸࡳΖ‫؀‬᨜ልᄐઔߒ 56:327-337Ζ ‫ء‬ઔߒհཬढ‫ޗ‬ற੡‫؀‬᨜‫ؾ‬ছࢬঅ‫ژ‬ऱ 21 ଡᖫ᜸ (Manihot esculenta Crantz) ጟ଺ ঴ጟΰߓα Δଈ٣‫شܓ‬ນᖲᏺཷ‫ڍ‬ীࢤ (Random Amplified Polymorphic DNA; RAPD) ։ ՗ᑑ፾Δၞ۩ᖫ᜸ጟ଺ᙊႚࣴฆ։࣫Δ‫א‬൶ಘ‫ٺ‬ጟ଺ၴհᘣᒴᣂএΖհ৵٦ၞԫ‫אףޡ‬ ១໢‫ݧ‬٨ૹᓤ (Simple Sequence Repeat; SSR) ։՗ᑑ፾ၞ۩ጟ଺ᦸࡳΔ‫׌‬૞‫ؾ‬ऱ‫࣍ڇ‬ཚ ඨ౨ല 21 ଡᖫ᜸঴ጟΰߓαၞ۩‫ݙ‬٤‫چ‬೴։ᦸܑΖRAPD ᇢ᧭࿨࣠ࢬᛧ൓ऱ 156 ය‫ڍ‬ ীࢤׂ੄Δᆖᆢႃ։࣫Δ21 ଡᖫ᜸ጟ଺‫ױ‬೴։‫ګ‬੡ 4 ᆢΔ‫ߛࠎ༼א‬ጟ೶‫ە‬Ζۖ‫ א‬11 ิ intra-SSR ֧՗ၞ۩ᇢ᧭Δᛧ൓ 32 ය‫ڍ‬ীࢤׂ੄Δᆖ‫ط‬ຍࠄ‫ڍ‬ীࢤׂ੄ऱิ‫ٽ‬Δ‫פګױ‬ ‫چ‬ല 21 ଡᖫ᜸঴ጟΰߓαၞ۩‫ݙ‬٤ऱ೴։Δ᧩‫ ق‬SSR ։՗ᑑ፾‫ڇ‬ᖫ᜸ጟ଺ᦸࡳΔ੡ԫ ൎۖ‫ڶ‬ԺհՠࠠΖ. ᙯᔣෟĈᖫ᜸ΕᙊႚࣴฆΕጟ଺ΕᦸࡳΕນᖲᏺཷ‫ڍ‬ীࢤΕ១໢‫ݧ‬٨ૹᓤΖ. ⓜ 岏 ᖫ᜸੡Օ༰ઝ (Euphorbiaceae) ԫ‫سڣڍࢨڣ‬հᑷ൅‫܂‬ढΔದᄭ࣍ᕠ۫ୂতຝΕ‫֣֗ࢮ್چذ‬ ۫ऱࠅ್᎝ࣾੌ഑Δ੡ॺ੊२ᐼশࢮ࿛Ւᣦຆᒃ‫چ‬೴Δપ 5 Ꮩ‫א‬ՂԳՑ‫ۍ‬։հ 60 ऱᑷၦࠐᄭ (FAO and IFAD 2000)Ζ‫ڼ‬؆Δᖫ᜸ٍ੡ૹ૞౨ᄭ‫܂‬ढΔ૿ኙ‫ؾ‬ছ٤෺౨ᄭ‫ٲ‬ᖲֲዬᣤૹΔ৺Ꮑၲ࿇‫س‬ᔆ ౨ᄭΔᖫ᜸ፖ‫ۏد‬Ε‫ز‬ᢑ࿛ઃ᥆࣍ᖸృᣊ‫܂‬ढΔຍࠄ‫܂‬ढຘመ࿇Ꭷመ࿓Δ‫سױ‬ข‫س‬ᔆԬᔧΰ಺壄αΔ ࠎ‫܂‬ᗏறࢨፖ޳ई෗‫ٽ‬৵Δ༼ࠎ޳߫ࠌ‫( ش‬Lin 2007)Ζ ᖂृམ༉๨‫ػ‬ᔆᐋ‫ڻ‬ऱ‫פٵ‬ฆዌᎧై (isozyme) ಾኙᖫ᜸ၞ۩ᦸࡳ (Hussain et al. 1987; Ramirez et al. 1987; Lefevre ϟ Charrier 1993)Δ‫ࡳᦸࠎױ࣍ૻ࠹܀‬ऱᙊႚᑑ፾ഗ‫ڂ‬ஆ‫ૻڶ‬Δࢬ‫א‬ข ‫س‬ऱ‫ڍ‬ীࢤլജۖྤऄၞ۩‫ڶ‬யऱᦸࡳ೴։Ζ‫ ࣍ط‬DNA ᐋ‫ࠎ༼ױڻ‬Օၦऱ։՗ᑑ፾ᑇ‫ؾ‬Δ‫ྤڼڂ‬ ᓵၞ۩ᖫ᜸ᦸࡳࢨᙊႚࣴฆ։࣫Δຟਢৰ‫ړ‬ऱՠࠠ (Beeching et al. 1993)Ζམᆖ‫࣍شܓ‬ᖫ᜸ऱ։՗ ᑑ፾‫ڶ‬ΚRFLP (Restriction Fragment Length Polymorphism) (Fregene et al. 1994)ΕRAPD (Random Amplified Polymorphic DNA) (Marmey et al. 1994; Wong et al. 1997)ΕAFLP (Amplifided Fragment Length Polymorphism) ࿛ (Roa et al. 1997; Wong et al. 1998; Chavarriaga-Aguirre et al. 1999; Elias et 1. 2. 3. 4.. ۩ਙೃልᄐࡡ୉ᄎልᄐᇢ᧭ࢬઔߒ໴‫ܫ‬ร 2306 ᇆΖ൷࠹ֲཚΚ96 ‫ ڣ‬12 ִ 15 ֲΖ ۩ਙೃልᄐࡡ୉ᄎልᄐᇢ᧭ࢬ‫܂‬ढጟ଺ิ‫ܗ‬෻ઔߒ୉Εልᄐᇢ᧭ࢬࢬ९Ζ ࠅ੊Օᖂ‫س‬ढઝ‫ߓݾ‬೫ඒ඄Ζ ຏಛ‫ृ܂‬Δሽ՗ၡٙΚ[email protected]ΙႚటᖲΚ(04)23331673Ζ.

(2) 328. ‫؀‬᨜ልᄐઔߒ! ร 56 ࠴! ร 4 ཚ. al. 2000)Ζ‫ڇ‬ຍࠄ DNA ։՗ᑑ፾խΔSSR ੡ለ௽௘ऱ։՗ᑑ፾Δࠡᙊႚᖲࠫ‫׌‬૞ਢ௅ᖕ೏࿛‫س‬ढ ࠠ‫ڶ‬ৰ೏ֺࠏऱ DNA ૹᓤ‫ݧ‬٨Δլ‫ڇ܀‬լ‫ٵ‬ढጟၴૹᓤ‫ݧ‬٨ऱᑇ‫ڶؾ‬ৰՕᙊႚ᧢ฆΔ‫ڇ‬լ‫ٵ‬঴ጟ ၴٍ‫ڶ‬ৰՕऱ஁ฆΖSSR ։՗ᑑ፾Ծ‫ױ‬։੡ࠟጟᣊীΔ௅ᖕፋ‫ٽ‬Ꭷైຑ᠙֘ᚨ (Polymerase Chain Reaction; PCR) ֘ᚨհขढլ‫ٵ‬Δૉ᥆࣍ૹᓤ‫ݧ‬٨փขढհឩᏺΔጠ੡ intra-SSRΙ۟࣍ૹᓤ‫ݧ‬٨հ ၴऱขढឩᏺΔጠ੡ inter-SSR ࢨ ISSRΖᖂृଚ࿇෼ᖫ᜸ጟ଺ࠠ‫ ڶ‬GA ១໢ૹᓤ‫ݧ‬٨ (GA)14 (Chavarriaga-Aguirre et al. 1998) Ζ ‫ ڼ ڂ‬ഏ Ꮎ ᑷ ൅ ል ᄐ խ ֨ (International Center for Tropical Agriculture; CIAT) ঁಾኙᖫ᜸ᚨ‫ ش‬SSR ၞ۩ᙊႚࣴฆ։࣫ፖጟ଺ᦸࡳ (Chavarriaga-Aguirre et al. 1998, 1999; Roa et al. 2000)Ζ ‫࣍ط‬ᖫ᜸‫܂ױ‬੡౨ᄭ‫܂‬ढΔૹ૞ࢤലֲ墿༼֒Ζ‫ء‬ઔߒհ‫ؾ‬ऱএಾኙ‫؀‬᨜‫ؾ‬ছࢬঅ‫ژ‬ऱ 21 ଡ ੴ၆ऱᖫ᜸ጟ଺঴ጟΰߓα Δ٣‫ױشܓ‬ข‫س‬Օၦය൅ऱ RAPD ։՗ᑑ፾Δၞ۩ᖫ᜸ጟ଺հᙊႚࣴฆ ৫։࣫Δ‫א‬൶ಘ‫ٺ‬ጟ଺ၴհᘣᒴᣂএΙհ৵٦ၞԫ‫ אޡ‬intra-SSR ։՗ᑑ፾Δၞ۩ጟ଺ᦸࡳΔ‫׌‬૞ ‫ؾ‬ऱ‫࣍ڇ‬ཚඨ౨ലࢬ‫ڶ‬ऱ 21 ଡጟ଺ຟ‫אױ‬ၞ۩‫ݙ‬٤ऱ೴։ᦸܑΔ‫א‬೚੡ጟ଺অߛհࠉᖕΖ. 㧟㠨咖㡈㽤 ֻྏങ‫ۏ‬Մफ़! ‫ء‬ᇢ᧭ࢬආ‫ش‬հᖫ᜸ጟ଺এঅ‫ࣟ؀࣍ژ‬೴ልᄐ‫ߜޏ‬໱Δ‫ץ‬ਔΚ‫؀‬তߛ 1-7 ᇆΕ୕ߺᙇ 1 ᇆΕ௻ ࣤ‫ג‬Εกֵ᜸ΕHicranΕRotiΕLunclnanΕBitterΕChirgwiΕVeipueenΕGreen twigΕBlack twigΕTuraiΕ MatnenotΕMedan ࿛Δ٥ૠ 21 ଡ঴ጟΰߓα Ζ ჌ّࣧ‫ې‬አߤ! ᓳ਷ႈ‫ץؾ‬ਔΚᆺ‫ۥ‬Εᆺ౧‫ۥ‬Εᆺਲ਼‫ۥ‬Εᆺׂ९ЯᐈֺଖΕ๓໮Ε։֭ᑇΕ௓೏࿛Ζ ങ‫ۏ‬វૄЯ௡ DNA ̝ප‫!פ‬ ‫׌‬૞೶‫ ە‬Doyle ϟ Doyle (1990) ࢬ༼հֱऄၞ۩ຝٝଥ‫אޏ‬ဇ࠷ཬढ᧯ഗ‫ ิڂ‬DNAΖ࠷પ 0.25 g ཬ௓ኊᆺΔ‫෈א‬ኪེઔᗣ৵߰ຒ‫ף‬Ե 500 PL բቃᑷ۟ 60кऱ DNA ဇ࠷ᒷᓢ෈Δᆜ࣍ 60к ֽ௮ 1 ՛ழΔࠀၴᄢᄀ೯Ζ࠷‫נ‬ᠦ֨ጥᙩᆜ‫ܐ‬থ۟৛ᄵ৵Δ‫ א‬14,000 rpm ᠦ֨ 5 ։ᤪΔ࠷Ղ堚෈ ଙ۟‫׼‬ԫᄅऱ 1.5 mL ՛ᠦ֨ጥխΔࠀ‫ף‬Ե 500 PL chloroform / isoamylalcohol (24:1) ᄫ෈෗֌Δ٦ ‫ א‬14,000 rpm ᠦ֨ 20 ։ᤪࠌࠟ෈ઌ։ᠦΔ࠷Ղ堚෈‫ף‬Ե 300 PL -20кऱ isopropanol ‫ިא‬ᖸ DNAΖ ‫ א‬14,000 rpm ᠦ֨ 10 ։ᤪΔଙൾՂ堚෈Δ‫ף‬Ե 1 mL wash buffer (75% ethanol, 10 mM ammonium acetate) ෗֌Δ࣍ 14,000 rpm ᠦ֨ 10 ։ᤪ৵ଙൾՂ堚෈Δല DNA ިᖸၞ۩೓ᛟ৵‫ף‬Ե 50 PL ྤပ ֽᄫᇞΔࠀ‫ף‬Ե RNase (10 ug/mL) ࣍ 37кՀ֘ᚨ 30 ։ᤪΖࢬࢼ൓ऱ DNA ݁ᆖ OD ଖྒྷࡳፖሽࣺΔ ‫ ܑܒא‬DNA ऱᖺ৫ፖ঴ᔆᛀྒྷΖ RAPD ྏរ! ‫ء‬ઔߒ٥ආ‫ ش‬14 ଡນᖲ֧՗Δ‫ ܛ‬OPERON kit հ OPC 1, OPC 5, OPG 2, OPG 8, OPG 13, OPG 18, OPO 10, OPO 13, OPO 20, OPW 2, OPW 4, OPW 6, OPW 13, OPY 1 ࿛ΖPCR ֘ᚨএ೶‫ ە‬Oard & Dronavalli (1992) ฃ‫܂‬ଥ‫ ޏ‬Δ‫ء‬ᇢ᧭֘ᚨ᜔᧯ᗨ੡ 30 ȝLΔփܶ 1.2 unit Taq polymerase (Promega)Ε 1X PCR buffer (50 mM KCl; 10 mM Tris-HCl, pH 9.0; 0.1% Triton 100)Ε2.5 mM MgCl2Ε200 ȝM dNTPΕ0.2 ȝM primer ֗ 50 ng template DNAΖPCR ֘ᚨএ࣍ Perkin Elmer Cetus Thermal Cycler 9600 ၞ۩Δ֘ᚨᄵ৫යٙ๻ࡳ੡ছ 2 ༛ᛩΚ94кΔ3 minΙ40кΔ1 min 20 secΙ72кΔ1 min 30 secΖհ ৵ᥛ‫ א‬94кΔ1 minΙ40кΔ1 minΙ72кΔ2 min ऱ֘ᚨᄵ৫යٙၞ۩ 43 ଡ༛ᛩΖ່৵ᆜ࣍ 72кΔ.

(3) ᖫ᜸ጟ଺։՗ᑑ፾ፖᙊႚࣴฆ৫! 329. 5 minΖ֘ᚨ‫ګݙ‬৵۞೯૾ᄵঅ‫ ࣍ژ‬4кՀΖᏺཷขढհೠྒྷ‫ א‬2% agarose ‫ ڇ‬1X TAE buffer (40 mM Tris acetate, pH 8.0; 1 mM EDTA) ၞ۩ DNA ሽࣺΔࠀ‫ א‬DNA molecular weight marker XVI (250 bp ladder)ΰRocheΔᐚഏα೚੡։՗ၦᑑ፾Δሽࣺᑒএආ‫ ش‬Mupid-2ΰCosmoΔֲ‫ء‬α Δሽᚘ๻ࡳ 100 ٗ௽Δᆖሽࣺ 20-30 ։ᤪ࿨‫ޔ‬৵ᆜ࣍ 0.5 mg/mL ऱ ethidium bromide ਩‫ ۥ‬10-20 ։ᤪΔ‫ א‬UV ٠ᛀ ီ agarose ᓄ᧯Ղऱ DNA ‫ڍ‬ীࢤׂ੄Δࠀᅃઌ֗ဿ‫ژ‬ᐙቝ࣍ IS 2000 Digital Imaging SystemΰAlpha Innotech CorporationΔભഏαΔ‫א‬ၞ۩৵ᥛऱᇷற։࣫Ζ ႃᆢ։࣫এലՂ૪ဿ‫ ࣍ژ‬IS 2000 Digtal Imaging System խऱ RAPD ขढሽࣺᐙቝ TIF ᚾΔ‫א‬ GelCompar ຌ᧯ (Applied Maths BVBA) ലሽࣺය൅࿨࣠᠏ང੡Ѐ/Ёীኪհᇷறী‫ڤ‬Δ٦‫֮אף‬஼ ๠෻᠏‫ ګ‬1/0 հᇷறী‫ڤ‬Ζ࣍ SAS ຌ᧯խᐷᐊ࿓‫ڤ‬Δല‫ ڼ‬1/0 ऱᇷறᚾΔ‫ א‬Jaccard's coefficient (Jaccard, 1908) ੡ഗ៕Δ٦‫ א‬SAS ຌ᧯հ CLUSTER procedurce UPGMA ၞ۩ႃᆢ։࣫Ζ SSR ྏរ! ‫ء‬ઔߒ٥ආ‫ ش‬13 ଡ SSR ֧՗Δ‫ ܛ‬SSRY9ΔSSRY11ΔSSRY12ΔSSRY14ΔSSRY25ΔSSRY49Δ SSRY63ΔSSRY68ΔSSRY75ΔSSRY76ΔSSRY91ΔSSRY93ΔSSRY106 (Chavarriaga-Aguirre et al. 1998)Ζ PCR ֘ᚨএ೶‫ ە‬Litt ϟ Lutty (1989) ฃ‫܂‬ଥ‫ޏ‬Δ‫ء‬ᇢ᧭֘ᚨᄫ෈᜔᧯ᗨ੡ 30 ȝLΔփܶ 1.2 unit Fast Start Taq DNA polymerase (Roche)Ε1 Ø PCR buffer (Roche)Ε2.5 mM MgCl2Ε200 ȝM dNTPΕ2.5 ȝM primer ֗ 50 ng template DNAΖPCR ֘ᚨএ࣍ Perkin Elmer Cetus Thermal Cycler 9600 ၞ۩Δ֘ᚨ ᄵ৫යٙ๻ࡳ੡ร 1 ༛ᛩΚ94кΔ3 minΙհ৵ᥛ‫ א‬94кΔ1 minΙ53кΔ2 minΙ72кΔ2 min ऱ֘ ᚨᄵ৫යٙၞ۩ 30 ଡ༛ᛩΖ່৵ᆜ࣍ 72кΔ5 minΖ֘ᚨ‫ګݙ‬৵۞೯૾ᄵঅ‫ ࣍ژ‬4кՀΖᆖ PCR ֘ᚨᏺཷհ DNA ขढΔ‫ א‬3.25%ऱ agarose ‫ ڇ‬1X TBE buffer խၞ۩ሽࣺ (Mupid-2 Cosmo)Δۖ৵ ലሽࣺᓄׂᆜ࣍ ethidium bromide խ਩‫ ۥ‬20-30 minΔ٦‫ א‬UV ٠ᛀီᓄ᧯Ղऱ DNA ឩᏺׂ੄Δࠀ ലᐙቝဿ‫ ࣍ژ‬IS2000 Digital Imaging System (Alpha Innotech Corporation)Ζ. 俟㨫 ፘᓥ჌ّࣧ‫ې‬አߤ! ‫ء‬ઔߒಾኙᖫ᜸ጟ଺ࢤणᓳ਷ऱ࿨࣠ᇡ‫ڕ‬। 1Ζ21 ଡᖫ᜸঴ጟΰߓαհᆺ‫ۥ‬ઃ੡ጸ‫ۥ‬Ιᆺ౧֗ ᆺਲ਼ऱᠱ‫ۥ‬ঞ‫ڶ‬દ‫֗ۥ‬ጸ‫ۥ‬հ։Δ༉ᆺ౧ፖᆺਲ਼‫ۥ‬հิ‫ٽ‬Δ‫ױ‬ലᖫ᜸ጟ଺೴։੡؄ጟীኪΔᆺ౧ፖ ᆺਲ਼ઃ੡દ‫ۥ‬ऱጟ଺‫ڍ່׭‬ᑇΔ٥‫ ڶ‬9 ଡΔ‫ץ‬ਔΚ‫؀‬তߛ 1Ε5Ε6Ε7 ᇆΔ‫ߺ୕֗א‬ᙇ 1 ᇆΕกֵ ᜸ΕBitterΕChirgwi ፖ MedanΖࠡ‫ڻ‬੡ᆺ౧ፖᆺਲ਼ઃ੡ጸ‫ۥ‬ऱጟ଺٥‫ ڶ‬8 ଡΔ‫؀ܛ‬তߛ 2Ε3Ε4 ᇆΔ ‫ ֗א‬HicranΕRotiΕVeipueenΕGreen twig ፖ TuraiΖᆺ౧ጸ‫ۖۥ‬ᆺਲ਼દ‫ۥ‬ऱጟ଺‫ڶ‬௻ࣤ‫ג‬ΕLunclnan ፖ MatnenotΖᆺ౧દ‫ۖۥ‬ᆺਲ਼ጸ‫ۥ‬ऱጟ଺‫ ڶ׽‬Black twigΖ ‫ڼ‬؆Δ। 1 ࿨ٍ࣠᧩‫ق‬ᖫ᜸‫ٺ‬঴ጟΰߓαհၴ‫ڇ‬ᆺׂऱ९ЯᐈֺΕ๓໮Ε։֭ᑇࠀྤለࣔ᧩‫ױ‬ ᙃܑऱ஁ฆΖ21 ଡᖫ᜸঴ጟΰߓαऱ௓೏։ؒᒤ໮Δؓ݁‫ ڇ‬131±23.84 cm ۟ 236±27.50 cm հၴΔ ೏ሒ 200 cm ‫א‬Ղऱᖫ᜸঴ጟΰߓα‫؀ڶ‬তߛ 4 ᇆΕBitterΕTuraiΕMatnenot ֗ Medan ࿛Ζ ᑕϡ RAPD ̶̄ᇾᄫซҖፘᓥ჌ࣧ᏷็‫ڡ‬ள̶‫!ژ‬ ‫ء‬ઔߒଈ٣ᆖ‫ط‬ᗴᙇᛧ൓Ա 14 ଡນᖲ֧՗Δຍࠄ֧՗‫׌‬૞௽‫ۥ‬੡‫ ڇױ‬21 ଡᖫ᜸ጟ଺ၴข‫ࠠس‬ ‫ڍڶ‬ীࢤ‫׊‬٦෼ࢤ᡹ࡳऱය൅ΖRAPD ᇢ᧭٥ૠข‫ س‬187 ය DNA ឩᏺׂ੄Δ։՗ၦտ࣍ 297-2951 bp հၴΔࠡխ‫ ڶ‬156 ය੡ࠠ‫ڍ‬ীࢤऱׂ੄Ζຍࠄ‫ڍ‬ীࢤׂ੄ᆖᆢႃ։࣫Δᅝࠟᆢհၴ່՛၏ᠦՕ࣍ Δ༼ࠎᛵᇞ‫ٺ‬঴ጟΰߓαհၴऱᘣᒴᣂএΔ‫א‬ 0.93 ழΔ‫᧩ࣔױ‬ല 21 ଡᖫ᜸ጟ଺೴։੡ 4 ᆢΰቹ 1α ‫܂‬੡৵ᥛߛጟհ೶‫ە‬Ζ.

(4) ‫؀‬᨜ልᄐઔߒ! ร 56 ࠴! ร 4 ཚ. 330. । 1. ᖫ᜸ጟ଺հࢤणᓳ਷ Table 1. The characteristics of cassava germplasm Color z Label. Varieties/lines. Leaf. Vein. Petole. Leaf ratio of length/width. Stalk Diameter (cm). No. of branch. Plant height (cm). 1. Tainanyu No.1. G. R. R. 0.7. 7.80±1.9. 3.3. 155±13.2. 2. Tainanyu No.2. G. G. G. 0.6. 7.20±0.4. 3.3. 133±7.4. 3. Tainanyu No.3. G. G. G. 0.7. 6.93±1.3. 3.3. 166±3.5. 4. Tainanyu No.4. G. G. G. 0.7. 6.80±1.2. 2.7. 227±40.4. 5. Tainanyu No.5. G. R. R. 0.7. 7.93±1.5. 2.3. 183±12.6. 6. Tainanyu No.6. G. R. R. 0.6. 6.28±1.2. 2.6. 151±10.6. 7. Tainanyu No.7. G. R. R. 0.6. 7.45±1.5. 3.0. 146±14.9. 8. Puli SEL.1. G. R. R. 0.7. 7.45±1.9. 3.5. 141±28.8. 9. Wu Chih Tsai. G. G. R. 0.6. 5.93±0.9. 2.7. 132±11.7. 10. Sweet. G. R. R. 0.6. 6.53±0.5. 3.3. 147±12.3. 11. Hicran. G. G. G. 0.6. 6.62±1.8. 3.2. 131±23.8. 12. Roti. G. G. G. 0.7. 5.80±1.6. 4.0. 139±8.9. 13. Lunclnan. G. G. R. 0.6. 7.93±2.8. 4.0. 182±31.8. 14. Bitter. G. R. R. 0.7. 7.50±2.4. 3.0. 236±27.5. 15. Chirgwi. G. R. R. 0.7. 6.15±1.6. 2.5. 171±5.0. 16. Veipueen. G. G. G. 0.8. 7.27±0.2. 2.3. 157±3.6. 17. Green twig. G. G. G. 0.6. 7.93±1.1. 1.7. 183±15.3. 18. Black twig. G. R. G. 0.6. 8.20±2.7. 3.7. 151±18.3. 19. Turai. G. G. G. 0.6. 9.50±0.7. 2.0. 225±35.4. 20. Matnenot. G. G. R. 0.6. 8.20±0.4. 3.3. 178±0.3. 21. Medan. G. R. R. 0.7. 7.25±2.5. 2.3. 206±31.5. z. G=Green; R=Red.. ᑕϡ SSR ̶̄ᇾᄫซҖፘᓥ჌ࣧ‫ݡ‬჌Ğրğᝥ‫!ؠ‬ ‫ء‬ઔߒএऴ൷ࠌ‫ ش‬Agarose ല၏ᠦઌ२ऱ‫ڍ‬ীࢤ intra-SSR ׂ੄ၞ۩೴։Δ੡ᇢ᧭‫ݾ‬๬հԫՕ ડధΰቹ 2α Δ‫ࢬ׊‬൓հ‫ڍ‬ীࢤׂ੄݁ᆖࡳ‫ݧ‬࿨࣠ᒔᎁࠡ intra-SSR հ‫ݧ‬٨Ζ‫ء‬ᇢ᧭ॣࡨ٥ૠࠌ‫ش‬Ա 19 ิ SSR ֧՗Δ‫ ڇױ܀‬21 ଡᖫ᜸ጟ଺ၴข‫ڍس‬ীࢤऱ֧՗ႛ 11 ิΰ। 2αΔขढ։՗ၦտ࣍ 255-1314 bp հၴΔ٥‫ױ‬ข‫ س‬32 ය‫ڍ‬ীࢤׂ੄Δࠡխ੷۟‫ױ‬ᛧ൓ 6 ය঴ጟΰߓα௽‫ڶ‬ऱറԫׂ੄Ζ ۖጵ‫ ٽ‬11 ิ intra-SSR ֧՗ࢬข‫س‬հ‫ڍ‬ীࢤׂ੄ऱิ‫ٽ‬Δ‫ױ‬ດ‫چޡ‬ല 21 ଡᖫ᜸঴ጟΰߓαၞ۩‫ݙ‬ ٤ऱ೴։Ζ ‫א‬Հঁಾኙ। 2 ऱઔߒ࿨࣠Δດ‫ޡ‬ၞ۩ጟ଺ᦸܑऱಘᓵΖଈ٣Δࠡխ‫ڶ‬؄ଡ঴ጟΰߓα‫ڶࠠٺ‬ ঴ጟ۞աऱ௽௘റԫය൅Δ‫ױڼڂ‬ፖࠡ塒ऱ 20 ଡ঴ጟΰߓαၞ۩೴ܑΰ‫ء‬ઔߒ٥ૠ 21 ଡ঴ጟΰߓαα Ζ ຍ؄ଡ঴ጟΰߓα੡ΚHicranΰ‫ז‬ᇆ 11α ΕLunclnanΰ‫ז‬ᇆ 13αΕMatnenotΰ‫ז‬ᇆ 20αΔ‫ ֗א‬Medan ΰ‫ז‬ᇆ 21α Ζ.

(5) ᖫ᜸ጟ଺։՗ᑑ፾ፖᙊႚࣴฆ৫! 331. ቹ 1. ᖫ᜸ጟ଺ RAPD ‫ א‬UPGMA ֱऄၞ۩հᆢႃ։࣫ᖫणቹΖ Fig. 1. Dendrogram of cluster analysis of cassava germplasm using UPGMA method based on RAPD.. Hicran ঴ጟΰߓα ΰ‫ז‬ᇆ 11αᚨ‫ ش‬SSRY12 ੡֧՗ิழΔ࣍ 255 bp ‫ۯ‬ᆜ๠ࠠ‫ڶ‬റ᥆ऱ௽௘റԫ ࢤය൅Δۖࠡ塒 20 ଡ঴ጟΰߓα࣍ᇠ‫ۯ‬ᆜઃྤය൅Δਚ‫ױ‬᎘࣐ല Hicran ፖࠡ،ᖫ᜸঴ጟΰߓα೴ ։ΖLunclnan ঴ጟΰߓαΰ‫ז‬ᇆ 13αᚨ‫ ش‬SSRY76 ੡֧՗ิழΔ࣍ 259 bp ‫ۯ‬ᆜ๠ࠠ‫ڶ‬റ᥆ऱ௽௘ റԫࢤය൅ΙMatnenot ঴ጟΰߓαΰ‫ז‬ᇆ 20αᚨ‫ ش‬SSRY49 ੡֧՗ิழΔ࣍ 290 bp ‫ۯ‬ᆜ๠ࠠ‫ڶ‬റ ᥆ऱ௽௘റԫࢤය൅ΙMedan ঴ጟΰߓα ΰ‫ז‬ᇆ 21αᚨ‫ ش‬SSRY12 ੡֧՗ิழΔ࣍ 400 bp ‫ۯ‬ᆜ๠ࠠ ‫ڶ‬റ᥆ऱ௽௘റԫࢤය൅Ζ ྥۖΔ૞ಾኙ‫ޢ‬ԫଡ঴ጟΰߓαຟ‫ࠩބ‬ᗑ௽റԫऱය൅ਢॺൄܺᣄऱΔ‫ڼڂ‬ൕࠃለՕ๵ᑓ‫چ‬ጟ ଺঴ጟΰߓαᦸࡳழΔൄᏁ૞‫ڍࠩش‬ଡ‫א‬Ղऱ֧՗ิ‫ٽ‬Ζ.

(6) ‫؀‬᨜ልᄐઔߒ! ร 56 ࠴! ร 4 ཚ. 332. । 2. ᖫ᜸ጟ଺հ intra-SSR ‫ڍ‬ীࢤׂ੄ Table 2. The intra-SSR polymorphic bands of 21 cassava germplasm accessions Variety/line z. Product size Primer SSRY 9. SSRY11. SSRY12. SSRY25. SSRY49. SSRY68. SSRY75. SSRY76. SSRY91 SSRY93. SSRY106 y z. (bp). 1. 2. 3. 4. 5. 6. 7. 8. 9 10 11 12 13 14 15 16 17 18 19 20 21. 286. -. y. -. +. -. -. -. -. +. -. -. - +. -. -. -. +. -. +. -. -. -. 271. +. +. -. +. +. +. +. -. + +. + +. +. +. +. -. +. -. +. +. +. 1314. +. +. -. +. -. -. +. +. - +. + +. +. +. +. +. +. +. +. -. -. 1201. +. -. +. +. +. +. -. +. + +. + +. +. +. +. +. +. +. +. +. +. 298. -. -. -. -. -. +. -. -. -. -. -. -. +. +. -. -. -. -. -. -. -. 276. -. -. -. -. -. -. -. -. -. -. +. -. -. +. +. -. -. -. -. -. -. 400. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. +. 302. -. +. -. -. +. +. +. -. +. -. +. -. +. -. -. -. -. -. -. -. -. 255. -. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -. -. -. -. -. -. 303. -. +. +. +. +. +. +. +. + +. - +. +. +. -. +. -. +. +. -. +. 284. +. -. +. +. -. -. -. -. + +. +. -. -. -. +. +. +. +. -. +. +. +. 341. +. -. -. +. +. +. +. -. -. -. -. -. +. -. +. -. -. +. -. 309. +. +. -. +. +. +. +. +. + +. -. + +. +. -. +. -. +. -. +. +. +. 290. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. -. +. -. 272. +. +. +. +. -. -. +. -. + +. + +. -. +. +. +. +. +. +. -. +. 325. -. +. -. +. +. +. +. +. +. -. - +. +. +. -. -. +. -. +. +. -. 302. +. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -. -. -. -. -. -. 291. -. +. -. +. +. +. +. +. + +. - +. +. +. +. -. +. -. +. +. +. 272. +. -. -. -. -. -. -. +. +. +. +. -. -. -. -. -. -. +. -. 259. +. +. +. +. +. +. +. -. - +. + +. -. +. +. +. +. +. +. -. +. 289. +. +. +. +. +. +. +. +. + +. + +. +. +. +. +. +. +. +. -. +. 271. +. -. -. -. -. -. -. -. - +. +. -. -. +. +. -. +. -. -. +. -. 305. -. -. -. -. -. -. -. -. -. +. -. +. +. +. -. -. -. -. -. -. 285. -. +. -. +. -. -. +. -. - +. - +. +. -. -. -. -. -. +. -. +. 272. +. -. +. -. +. +. -. +. +. -. +. -. -. +. +. +. +. +. -. +. -. 259. -. -. -. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -. -. -. -. 340. +. -. +. +. -. -. -. -. - +. - +. -. -. +. +. -. +. +. -. +. -. -. -. -. 326. +. -. +. +. +. +. -. -. + +. + +. -. +. +. +. +. +. +. -. -. 306. -. -. -. -. -. -. +. -. -. -. -. +. -. -. -. -. -. -. -. -. 290. +. -. +. +. +. +. -. +. + +. + +. -. +. -. -. -. +. +. -. +. 272. +. +. -. -. +. +. +. -. + +. + +. +. +. +. +. +. -. +. +. -. 258. -. -. -. -. -. -. -. -. -. +. -. -. -. -. -. -. -. -. -. +:band is present ; -:band is absent. Label of variety/line see as Table 1.. -. -. -.

(7) ᖫ᜸ጟ଺։՗ᑑ፾ፖᙊႚࣴฆ৫! 333. ቹ 2. 21 ଡᖫ᜸ጟ଺հ intra-SSR ᡯᓄሽࣺቹΖ Fig. 2. The intra-SSR electrophoretic profiles on agarose for 21 cassava germplasm accessions. (M:100 bp marker; 1-21: Label of variety/line was described in Table 1.). ༉‫ء‬ઔߒᖫ᜸঴ጟΰߓαऱᦸࡳۖߢΔଈ٣‫່א‬១໢ऱࠟଡ֧՗ิ‫ٽ‬੡ࠏΚ‫؀‬তߛ 1 ᇆΰ‫ז‬ᇆ 1α ᚨ‫ ش‬SSRY68 ੡֧՗ิΔ៶‫ ط‬302 bp ‫ۯ‬ᆜऱറԫࢤය൅Δբᆖ‫ױ‬ፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαխऱ 19 ଡ঴ጟΰߓαၞ۩೴ܑΔႛྤऄፖ Hicranΰ‫ז‬ᇆ 11α೴ܑΖ‫ط܀‬Ղ૪ಘᓵΔ‫ݺ‬ଚբᆖᛵᇞ Hicran ঴ጟΰߓαᚨ‫ ش‬SSRY12 ੡֧՗ิழΔ࣍ 255 bp ‫ۯ‬ᆜ๠ࠠ‫ࠡڶ‬റ᥆ऱ௽௘റԫࢤය൅Ζ‫ڼڂ‬ጵ‫ٽ‬ SSRY68 ֗ SSRY12 ࠟଡ֧՗ิΔ‫؀‬তߛ 1 ᇆፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαঁ‫ݙאױ‬٤‫چ‬೴։Ζ ‫א‬Ղ‫ٵ‬ᑌऱ঴ጟΰߓαᦸࡳ଺෻‫ױ‬ሎ‫؀ڇش‬তߛ 7 ᇆΖ‫؀‬তߛ 7 ᇆΰ‫ז‬ᇆ 7αᚨ‫ ش‬SSRY93 ੡ ֧՗ิΔ៶‫ ط‬306 bp ‫ۯ‬ᆜऱറԫࢤය൅Δբᆖ‫ױ‬ፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαխऱ 19 ଡ঴ጟΰߓα ၞ۩೴ܑΔႛྤऄፖ Lunclnanΰ‫ז‬ᇆ 13α೴ܑΖ‫ط܀‬Ղ૪ಘᓵΔ‫ݺ‬ଚբᆖᛵᇞ Lunclnan ঴ጟΰߓα ᚨ‫ ش‬SSRY76 ੡֧՗ิழΔ࣍ 259 bp ‫ۯ‬ᆜ๠ࠠ‫ڶ‬റ᥆ऱ௽௘റԫࢤය൅Ζ‫ڼڂ‬ጵ‫ ٽ‬SSRY93 ֗ SSRY76 ࠟଡ֧՗ิΔ‫؀‬তߛ 7 ᇆፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαঁ‫ݙאױ‬٤‫چ‬೴։Ζ ۟࣍‫؀‬তߛ 6 ᇆΰ‫ז‬ᇆ 6αΔឈྥՈ‫ࠩش׽‬Աࠟଡ֧՗ิ‫ٽ‬Δ‫ܑܒࡳᦸ܀‬ऱൣ‫ݮ‬ঞለ੡ᓤᠧΚ ‫؀‬তߛ 6 ᇆᚨ‫ ش‬SSRY11 ੡֧՗ิழΔ៶‫ ط‬298 bp ‫ۯ‬ᆜऱറԫࢤය൅Δբᆖ‫ױ‬ፖࠡ، 18 ଡ঴ጟΰߓα ၞ۩೴ܑΔ‫ྤ܀‬ऄፖ Lunclnanΰ‫ז‬ᇆ 13α ΕBitterΰ‫ז‬ᇆ 14α೴ܑΖ‫ ࣍ط‬Lunclnan ঴ጟΰߓαᚨ‫ش‬ SSRY76 ੡֧՗ิழΔ࣍ 259 bp ‫ۯ‬ᆜ๠ࠠ‫ڶ‬റ᥆ऱ௽௘റԫࢤය൅Ζ‫ؾڼڂ‬ছ‫ ໍ׽‬Bitterΰ‫ז‬ᇆ 14α ྤऄፖ‫؀‬তߛ 6 ᇆ‫܂‬೴ܑΔᅝ‫ݺ‬ଚ‫ڃ‬ᙰ٦‫ڻ‬ᛀီ। 2 ऱઔߒ࿨࣠Δ࿇෼‫ڇ‬ᚨ‫ ش‬SSRY11 ੡֧՗ิऱ ‫ٵ‬ழΔBitterΰ‫ז‬ᇆ 14α࣍ 1,314 bp ፖ 276 bp ๠ٍࠠ‫ڶ‬ය൅Δۖ‫؀‬তߛ 6 ᇆঞྤය൅Ζጵ‫ٽ‬Ղ૪Δ ‫؀‬তߛ 6 ᇆᖫ᜸঴ጟΰߓα౨ജፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαሒࠩ‫ݙ‬٤‫چ‬೴։ΔឈྥՈ‫ࠩش׽‬Աࠟ ଡ֧՗ิ‫ٽ‬ΰSSRY11 ֗ SSRY76α Δ‫ࡳᦸ܀‬ऱ‫ܑܒ‬೴഑থሀ။Ա؄ଡය൅‫ۯ‬ᆜΔ‫ ܛ‬SSRY11 ֧՗ิ ऱ 1,314 bpΕ298 bpΕ276 bp ፖ SSRY76 ֧՗ิऱ 259 bp ‫ۯ‬ᆜΔࢬ‫ܑܒࡳᦸא‬ൣ‫ݮ‬ለ੡ᓤᠧΖ ‫ء‬ઔߒխ࿇෼ SSRY11 ਢԫଡৰ‫ڶ‬ய෷ऱ֧՗ิΖ༉Ղ૪հᦸࡳመ࿓Δངଡߡ৫‫ א‬Bitterΰ‫ז‬ ᇆ 14α੡‫ࠐߡ׌‬઎ΔࠡኔՈਢ‫ ܑܒ‬Bitterΰ‫ז‬ᇆ 14α‫ױ‬ፖࠡ، 20 ଡᖫ᜸঴ጟΰߓαሒࠩ‫ݙ‬٤‫چ‬೴.

(8) 334. ‫؀‬᨜ልᄐઔߒ! ร 56 ࠴! ร 4 ཚ. ։հᦸࡳመ࿓Ζೈ‫ڼ‬հ؆ΔᤉᥛലՂ૪ SSRY11 ੡֧՗ิհᦸࡳઔߒ࿨࣠࢏‫ۼ‬Δࠡ 276 bp ‫ۯ‬ᆜ‫׽‬ ‫ ڶ‬Hicranΰ‫ז‬ᇆ 11α ΕBitterΰ‫ז‬ᇆ 14α֗ Chirgwiΰ‫ז‬ᇆ 15α Ζ‫ ࣍ط‬Hicran ঴ጟΰߓαᚨ‫ ش‬SSRY12 ੡֧՗ิழΔ࣍ 255 bp ‫ۯ‬ᆜ๠ࠠ‫ࠡڶ‬റ᥆ऱ௽௘റԫࢤය൅Ζۖ‫ ׊‬SSRY11 ֧՗ิऱ 298 bp ‫ۯ‬ᆜΔ Chirgwiΰ‫ז‬ᇆ 15αྤය൅Δۖ Bitterΰ‫ז‬ᇆ 14α‫ڶ‬ය൅Δ࣍ਢԾ‫ڍױ‬೴։‫נ‬ԫଡ঴ጟΰߓαChirgwi ΰ‫ז‬ᇆ 15α Ζ ‫ڇ‬ၞ۩ጟ଺ᦸࡳழΔ‫ݺ‬ଚཚඨ‫່֟אױ‬ऱ֧՗ᑇ‫ؾ‬Δሒ‫່ګ‬Օऱᦸࡳய෷Ζ‫ڼڂ‬Δኲ۟‫ؾ‬ছ੡ ַΔբ‫ױ‬ല԰ଡ঴ጟΰߓα໢ᗑ‫ط‬٤ຝ 21 ଡ঴ጟΰߓαխᦸܑ‫ࠐנ‬Δࢬ‫֧ࠩشܓ‬՗ิ‫ ڶ׽ٽ‬6 ଡΚ SSRY11ΕSSRY12ΕSSRY49ΕSSRY68ΕSSRY76ΕSSRY93Ζ 塒‫ڼ‬ᣊංΔᆖ‫ ط‬11 ิ֧՗ኙࢬข‫س‬ऱ 32 ය DNA ນᖲᏺ༏ׂ੄‫ױ‬ല 21 ଡᖫ᜸঴ጟΰߓαԫ ԫ೴։‫ࠐנ‬Ζ. 岝嵥 ᦸܑጟ଺່១໢Εऴ൷ऱֱ‫ڤ‬ਢ‫ط‬؆ᨠࢤणࠐ‫ܑܒ‬Δ‫ء‬ઔߒଈ٣ቫᇢ‫א‬ᖫ᜸ऱᆺΕᆺ౧Εᆺਲ਼ ऱᠱ‫ۥ‬Δ‫֗א‬ᆺׂऱ९ЯᐈֺΕ๓໮Ε։֭ᑇፖ௓೏࿛ࢤणΔၞ۩ॣ‫ޡ‬ऱ঴ጟΰߓαᦸࡳΖ࿨࣠᧩ ‫ٺق‬঴ጟΰߓαၴ‫ڇ‬ᆺ౧‫ۥ‬Εᆺਲ਼‫ۥ‬ຍࠟଡႈ‫ؾ‬ឈࠠ‫ڶ‬ለࣔ᧩‫ױ‬ᙃܑऱ஁ฆΔ‫܀‬սྤऄ‫אش‬೴։ 21 ଡᖫ᜸ጟ଺Ζ‫ڼ‬؆ΔຝٝᑇၦࢤणΔ‫ڕ‬๓໮Ε։֭ᑇΕ௓೏࿛ΔೈԱ࠹ഗ‫ڂ‬ᐙ᥼հ؆Δ்ഛᛩቼՈ ‫ױ‬౨֧ದࢤण।෼஁ฆΔਚᑇၦࢤणᓳ਷Δ‫ؘ‬ႊ‫ٵڇ‬ԫ்ഛᛩቼՀࢬᛧᇷறΔթ౨ၞ۩঴ጟᦸࡳΖ DNA ᐋ‫ڻ‬ऱ։՗ᑑ፾‫࣍ط‬լ࠹ᛩቼᐙ᥼Δ‫شࠌױ׊‬ऱᑑ፾‫ۯ‬ᆜᑇ‫ڍؾ‬Δ‫ڼڂ‬ԫ౳๯ᎁ੡ਢၞ ۩ጟ଺ᦸࡳऱߜ‫ړ‬ՠࠠΖ‫ءڇ‬ઔߒխΔ‫ݺ‬ଚࠌ‫ش‬Ա RAPD ፖ intra-SSR ࠟጟ։՗ᑑ፾Δ࿨࣠࿇෼ RAPD ឈྥ‫אױ‬ข‫س‬ለ‫ڍ‬ऱ‫ڍ‬ীࢤය൅Δ‫܀‬૞‫ࠩ܂‬ല 21 ଡᖫ᜸ጟ଺ၞ۩‫ݙ‬٤ऱ೴ܑথৰܺᣄΙઌ ኙऱΔ‫ א‬11 ิ intra-SSR ֧՗ၞ۩ᇢ᧭Δឈႛ‫ױ‬ᛧ൓ 32 ය‫ڍ‬ীࢤׂ੄Δ‫܀‬ᆖ‫ط‬ຍࠄ‫ڍ‬ীࢤׂ੄ऱ ิ‫ٽ‬Δথ‫چפګױ‬ല 21 ଡᖫ᜸঴ጟΰߓαၞ۩‫ݙ‬٤ऱ೴։Ζ൶ߒࠡ଺‫ڂ‬Δທ‫ڼګ‬࿨࣠ऱ‫׌‬૞଺‫ڂ‬ ੡ RAPD ࢬࠌ‫ش‬ऱ֧՗ਢນᖲऱ‫ݧ‬٨Δ։ཋ‫ཬڇ‬ढ᧯ഗ‫ิڂ‬խΖ‫܀‬ਢ intra-SSR ऱ‫ݧ‬٨ഗ៕ਢઌ‫ٵ‬ ऱΔ‫׽‬ਢᙊႚᖲࠫՂຍࠄ DNA ૹᓤ‫ݧ‬٨ऱᑇ‫ؾ‬Δ‫ڇ‬լ‫ٵ‬঴ጟΰߓαၴ‫஁ڶ‬ฆΖ‫ءڼڂ‬ઔߒऱᆖ᧭Δ ‫ך‬։᧩‫ ق‬intra-SSR ։՗ᑑ፾‫ڇ‬ᖫ᜸ጟ଺ᦸࡳΔ੡ԫൎۖ‫ڶ‬ԺհՠࠠΖ ‫܀‬༉൶ಘጟ଺ᙊႚࣴฆऱᨠរۖߢΔ‫ء‬ઔߒආ࠷ RAPD ࿨࣠ၞ۩ᆢႃ։࣫Δ‫ڂ‬੡ RAPD ‫ױ‬ข ‫س‬ऱ‫ڍ‬ীࢤ೏Δኙ࣍ᐖऑ൶ಘഗ‫ิڂ‬հၴऱᙊႚ᧢ฆፖᘣᒴᣂএΔᄎለ੡ᔞ֊Ζ‫ء‬ᇢ᧭հ RAPD ࿨࣠Δ‫ܧ‬෼‫ٺנ‬ᖫ᜸঴ጟΰߓαၴऱᙊႚ၏ᠦΔ‫ߛ࣍ܗڶ‬ጟᘣ‫ء‬հᙇᖗΔፖᠧٌՠ‫܂‬հၞ۩Δ‫ףױ‬ ຒᚌߜᠧٌ৵ᇔհᛧ൓Δኙ࣍༼ࣙᖫ᜸հߛጟய෷Δࠠ‫ڶ‬ኔ‫ش‬հᏝଖΖ ᆖ‫אط‬Ղ૪࿨࣠‫ױ‬वΔSSR ։࣫ࢬೠྒྷऱ‫ؾ‬ᑑ‫ݧ‬٨հঅ‫ࢤښ‬ለ೏Δ‫׊‬ຏൄॺ‫פ‬౨ࢤഗ‫ڂ‬Δࢬ ‫א‬լႛ‫ݧ‬٨ऱૹᓤࢤ೏Δ‫ݧڼ‬٨Ոլ࣐࠹ᛩቼᐙ᥼ۖ‫᧢ޏ‬Δ‫ڼڂ‬։࣫࿨࣠ለ᡹ࡳΔࢬ‫ شܓא‬SSR հ֧՗ิΔၞ۩ PCR ֘ᚨΔࠡࢬᏺ༏հය൅ຏൄֺ RAPD ໢ొΔ‫׊‬٦෼ࢤ೏Δ‫אױ‬ᚦᇖ RAPD ٦ ෼ࢤ஁հ౒រΔ‫ ڼڂ‬SSR ਢૹ૞ऱ঴ጟᦸܑՠࠠհԫΖ. 崛嶬 ‫ء‬ᇢ᧭ᖫ᜸ጟ଺‫ޗ‬றࢭ፞ছ‫ࣟ؀‬೴ልᄐ‫ߜޏ‬໱‫܂‬ढ‫ߜޏ‬ᓰছઔߒ୉ଫᓰ९‫ޕ‬ᘋၞ(բಯٖ)༼ ࠎΔ௽‫ڼ‬ტ᝔Ζ‫ء‬ઔߒࢭልࡡᄎૠ྽ᆖ၄֭਍Δ௽‫ع‬᝔‫ݵ‬Ζ.

(9) ᖫ᜸ጟ଺։՗ᑑ፾ፖᙊႚࣴฆ৫! 335. ㆤ䞷㠖䘊 (Literature Cited) Beeching, J. R., P. Marmey, M. C. Gavalda, M. Noirot, H. R. Haysom, M. A. Hughes, and A. Charrier. 1993. An assessment of genetic diversity within a collection of cassava (Manihot esculenta Crantz) germplasm using molecular markers. Ann. Bot. 72:515-520. Chavarriaga-Aguirre, P., M. M. Maya, M. W. Bonierbale, S. Kresovich, M. A. Fregene, J. Tohme, and G. Kochert. 1998. Microsatellites in cassava (Manihot esculenta Crants):discovery, inheritance and variability. Theor. Appl. Genet. 97:493-501. Chavarriaga-Aguirre, P., M. M. Maya, J. Tohme, M. C. Duque, C. Iglesias, M. W. Bonierbale, S. Kresovich, and G. Kochert. 1999. Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in cassava core collection and to assess the usefulness of DNA-based markers to maintain germplasm collections. Mol. Breed. 5:263-273. Doyle, J. J., and J. L. Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. Elias, M., O. Panaud, and T. Robert. 2000. Assessment of genetic variability in a traditional cassava (Manihot esculenta Crantz) farming system, using AFLP markers. Heredity 85:219-230. FAO and IFAD. 2000. Cassava medium-term outlook. in: The World Cassava Economy: Facts, Trends and Outlook. FAO and IFAD press, Rome. Fregene, M. A., J. Vargas, F. Angel, J. Thome, R. A. Asiedu, M. O. Akorada, and W. M. Roca. 1994. Chloroplast DNA and nuclear ribosomal DNA variability in cassava (Manihot esculenta Crantz) and its wild relatives. Theor. Appl. Genet. 89:719-727. Hussain, A., W. Bushuk, H. Ramirez, and W. M. Roca. 1987. Identification of cassava (Manihot esculenta Crantz) cultivars by electrophoretic patterns of esterase isozyme. Seed Sci. Tech. 15:19-22. Jaccard, P. 1908. Nouvelles recherches sur la distribution florate. Bull. Soc. Vaudoise Sa. Not. 44:223-270. Lefevre, F., and A. Charrier. 1993. Heredity of seventeen isozyme oci in cassava (Manihot esculenta Crantz). Euphytica 66:171-178. Lin, C. Y. 2007. Prospects of the international promotion for bio-energy crop.. Forestry Research. Newsletter 14(3):35-40. Litt, M., and J. A. Lutty. 1989. A hypervariable microsatellite revealed by in vitro amplification of dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet. 44:397-401. Marmey, P., J. R. Beeching, S. Hamon, and A. Charrier. 1994. Evaluation of cassava (Manihot esculenta Crantz) germplasm collections using RAPD makers. Euphytica 74:203-209. Oard, J. H., and S. Dronavalli. 1992. Rapid isolation of rice and maize DNA for analysis by random primer PCR. Plant Mol. Biol. Rep. 10:236-241. Ramirez, H., A. Hussain, W. M. Roca, and W. Bushiuk. 1987. Isozyme electro-phenograms of sixteen enzymes in five tissues of cassava (Manihot esculenta Crantz) varities. Euphytica 36:39-48..

(10) 336. ‫؀‬᨜ልᄐઔߒ! ร 56 ࠴! ร 4 ཚ. Roa, A. C., M. M. Maya, M. C, Duque, J. Tohme, A. C. Allem, and M. W. Bonierbale. 1997. AFLP analysis of relationships among cassava and other Manihot species. Theor. Appl. Genet. 95:741-750. Roa, A. C., P. Chavarriaga-Aguirre, M. C. Duque, M. M. Maya, M. W. Bonierbale. C. Iglesias, and J. Tohme. 2000. Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) Microsatellites:allelic polymorphism and degree of relationship. Am. J. Bot. 87:1647-1655. Wong, H. L., H. H. Yeoh, S. H. Lim, and K. C. L. Looi. 1997. Design of primers for RAPD analyses of cassava, Manihot esculenta. Phytochemistry 46:805-810. Wong, H. L., H. H. Yeoh, and S. H. Lim. 1998. Customisation of AFLP analysis for cassava varietal identification. Phytochemistry 50:919-924..

(11) ᖫ᜸ጟ଺։՗ᑑ፾ፖᙊႚࣴฆ৫! 337. Genetic Diversity and Identification of Cassava Germplasm with RAPD and SSR1 Shu Chen2,4, Chung-Chen Fan3 and Chien-Yih Lin2 Abstract Chen, S., C. C. Fan, and C. Y. Lin. 2007. Genetic diversity and identification of cassava germplasm with RAPD and SSR. J. Taiwan Agric. Res. 56:327-337. The research aimed to identify 21 cassava (Manihot esculenta Crantz) germplasm accessions collected from Taiwan. The objectives were to understand genetic diversity among cassava germplasm accessions using RAPD and to identify each of them by intra-SSR. 21 accessions of cassava germplasm were clustered to 4 groups based on 156 polymorphic bands of RAPD. From the analysis of RAPD, it could provide as references for breeders, 32 polymorphic bands from 11 SSR primers were found and the 21 cassava varieties / lines could be differentiated completely using these intra-SSR polymorphic bands. This research revealed that the intra-SSR is a powerful molecular tool for the identification of cassava germplasm. Key words: Cassava (Manihot esculenta Crantz), Genetic diversity, Germplasm, Identification, RAPD, SSR.. 1. Contribution No.2306 from Agricultural Research Institute, Council of Agriculture. Accepted: December 15, 2007. 2. Assistance Researcher, Plant Germplasm Division, ARI; Director-general, ARI, Wufeng, Taichung, Taiwan, ROC. 3. Associate Professor, Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan, ROC. 4. Corresponding author, e-mail:[email protected]; Fax:(04)23331673..

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