行政院國家科學委員會補助專題研究計畫 V 成果報告
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電 壓 依 賴 性 鈉 離 子 通 道 與 上 皮 鈉 離 子 通 道 的 功 能 性 活 性 對 細 胞 電 行 為 影 響 的 可 能 機 制
計畫類別：V 個別型計畫 □整合型計畫
計畫編號：NSC 98－2320- B－006－027－MY3
執行期間：2009 年 08 月 01 日至 2012 年 07 月 31 日
執行機構及系所：國立成功大學生理研究所
計畫主持人：吳勝男
共同主持人：
計畫參與人員：許銘淳, 廖俞凱, 楊偉欣, 黃獻慶
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附件一
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國科會補助專題研究計畫成果報告自評表
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綜合以上的結果，我們認為在運動神經細胞，Flupirtine 可以視為遲緩性糾正鉀離子通道的
開啟狀態的阻斷劑。Flu 引起 IK(DR)的阻斷作用是不可能經由 NMDA 或 GABA 受體的接合
所引起的。而且 Flu 對鉀離子通道的作用並不是只局限在 M 型鉀離子通道。本論文對日後 止痛藥物的離子作用機制有重大幫助。
附件三
電 壓 依 賴 性 鈉 離 子 通 道 與 上 皮 鈉 離 子 通 道 的 功 能 性 活 性 對 細 胞 電 行 為 影 響 的 可 能 機 制
(NSC 98-2320-B-006-027-MY3)
結 案 報 告 中文摘要
Flupirtine (Flu)是一種 triaminopyridine 的衍生物，它是一種中樞作用非鴉片類的止痛劑。Flu 的離
子作用機制過去認為主要是和活化 M 型鉀電流(IK(M))有關。可是 Flu 是否會對運動神經細胞中的其它離
子電流有所作用則不甚清楚。在此份研究報告中，我們使用兩種不同類似運動神經元的細胞，並加以 測定這些細胞中 Flu 對鉀離子電流的影響。在 NSC-34 神經細胞中，細胞曝露在 Flu 時會減少遲緩性糾
正鉀離子電流(IK(DR))的幅度，同時合倂增加該電流的去活化。利用極小接和模式，Flu 引起 IK(DR)去活
化的解離常數為 9.8 µM。Linipirdine (10 µM), NMDA (30 µM) 或 gabazine (10 µM)皆無法逆轉 Flu 所改
變的電流去活化。Flu 的投與下會使得 IK(DR) 去活化曲線偏移至過極化電位，但對於斜率因子並無影
響。在 Flu 的存在下，IK(DR)累積性去活化也受提高。另外，Flu 也增加 IK(M)的強度，而且 IK(M)的活化
曲線會產生往左移的現像，但不會改變該電流的調空電荷。而在 NH108-15 神經細胞，Flu 也減少 IK(DR)
的強度以及增加 IK(DR)的去活化速率。綜合以上的結果，我們認為在運動神經細胞，Flu 可以視為遲緩
性糾正鉀離子通道的開啟狀態的阻斷劑。Flu 引起 IK(DR)的阻斷作用是不可能經由 NMDA 或 GABA 受
體的接合所引起的。而且 Flu 對鉀離子通道的作用並不是只局限在 M 型鉀離子通道。
關鍵詞: Flupirtine, 運動神經元, 遲緩性糾正鉀離子電流, M 型鉀電流, 電流動力學
Abstract
Flupirtine (Flu), a triaminopyridine derivative, is a centrally acting, non-opiate analgesic agent. The ionic mechanism of Flu actions is primarily related to the activation of M-type K+ current (IK(M)). However, whether Flu produces any effects on ion currents in motor neurons remains largely unknown. In this study, effects of Flu on K+ currents were explored in two types of motor neuron-like cells. Cell exposure to Flu decreased delayed rectifier K+ current (IK(DR)) amplitude with a concomitant raise in current inactivation in NSC-34 neuronal cells. Based on minimal binding scheme, the dissociation constant for Flu-mediated increase of IK(DR) inactivation rate was about 9.8 µM. However, neither linopirdine (10 µM), NMDA (30 µM), nor gabazine (10 µM) reversed Flu-induced changes in IK(DR) inactivation. Addition of Flu shifted the inactivation curve of IK(DR) to a hyperpolarized potential with no change in the slope factor. Cumulative inactivation for IK(DR) was elevated in the presence of this compound. Flu increased the amplitude of M-type K+ current (IK(M)) and produced a leftward shift in the activation curve of IK(M) with no apparent change in the gating charge of this current. In another neuronal cells (NG108-15), Flu reduced IK(DR) amplitude and enhanced the inactivation rate of IK(DR). Taken together, the results suggest that Flu acts as an open-channel blocker of delayed-rectifier K+ channels in motor neurons. Flu-induced block of IK(DR) presented herein is unlinked to binding to NMDA or GABA receptors and the effects of this agent on K+ channels are not limited to its action on M-type K+ channels.
Keywords: Flupirtine, motor neuron, delayed-rectifier K+ current, M-type K+ current, current kinetics
1. Introduction
Flupirtine (Flu) which belongs to a class of triaminopyridines is a centrally acting, nonopioid analgesic agent with muscle relaxing properties. It has been used for a wide variety of neurological disorders involving neuronal overexcitability including epilepsy and neuropathic pain [1]. In addition to its use in pain management, Flu was also reported to display muscle relaxant and anticonvulsant activity. Notably, this compound was previously demonstrated to be beneficial in treating the patients with human prion diseases [2, 3]. A recent study showed the ability of Flu to reduce the myotonic membrane hyperexcitability induced by a Cl- channel blocker [4].
Earlier studies have demonstrated that it might act as an antagonist of N-methyl-D-aspartate (NMDA) receptors [5, 6]. A recent report also showed the ability of Flu to facilitate the activity of GABAA receptors accompanied by increased stimulation of KV7 channels [7]. More importantly, it is accepted that Flu can bind to and activate voltage-gated K+ (KV) channels subtypes 7.2-7.5 (KV7.2-7.5). The increased activity of KV7.2, 7.3 and 7.5 channels generates the so-called M-type K+ current (IK(M)) which is a slowly activating and deactivating current suppressed by stimulation of muscarinic receptors [8-10]. Mutations of the KCNQ2 gene are notably involved in peripheral nerve hyperexcitability, a syndrome which is characterized by a spontaneous and continuous muscle overactivity [11]. However, whether Flu and its structurally related compounds can produce any effects on other type of KV channels remains unclear.
The NSC-34 cell is a hybridoma cell line derived from the fusion of neuroblastoma cells with mice spinal cord cells. These cells have attracted growing interest as a suitable model for the evaluation of effects of potential neuroprotective compounds against different insults including excitotoxins, mitochondrial toxin and oxidants [12, 13]. Previous observations in our laboratory have shown the presence of Na+-activated K+ channels functionally expressed in these cells [14].
In this study, we attempted to evaluate the possible effects of Flu and other related compounds on ion currents in NSC-34 neuroblastoma-spinal cord hybrid cells and NG108-15 neuronal cells. Of note, we found that in NSC-34 and NG108-15 neuronal cells, addition of Flu is capable of producing inhibitory actions on delayed-rectifier K+ current (IK(DR)) in a concentration- and state-dependent fashion. The elevation of IK(DR)
inactivation rate caused by this compound was further quantitatively characterized in this study. The major action of this compound on IK(DR) is thought to be through an open-channel mechanism. Findings from this
study indicate that the inhibition by Flu of IK(DR) in these neurons is not associated with a mechanism linked to the binding of either NMDA or GABA receptors and that the effects of Flu on K+ channels are not limited to its modification of M-type K+ channels.
2. Materials and Methods 2.1. Drugs and solutions
Flupirtine maleate (Flu; ethyl{2-amino-6-[(4-fluorobenzyl)amino]pyridine-3-yl}carbamate; Katadolon®), linopirdine, meclofenamic acid, 4-aminopyridine, tetraethylammonium chloride (TEA) and tetrodotoxin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Gabazine and N-methyl-D-aspartic acid (NMDA) was from Tocris Cookson, Ltd. (Bristol, UK). Agitoxin-2, apamin, iberiotoxin and margatoxin were obtained from Alomone Labs (Jerusalem, Israel). All culture media, fetal bovine serum, L-glutamine, trypsin/EDTA and penicillin-streptomycin were obtained from Invitrogen (Carlsbad, CA, USA). All other chemicals were obtained from regular commercial chemicals and of reagent grade.
The composition of the bath solution (i.e., normal Tyrode’s solution) was 136.5 mM NaCl, 5.4 mM KCl, 1.8 mM CaCl2, 0.53 mM MgCl2, 5.5 mM glucose, and 5.5 mM HEPES-NaOH buffer, pH 7.4. To record IK(DR) or IK(M), the recording pipettes were backfilled with a solution consisting of 130 mM K-aspartate, 20 mM KCl, 1 mM KH2PO4, 1 mM MgCl2, 3 mM Na2ATP, 0.1 mM Na2GTP, 0.1 mM EGTA, and 5 mM HEPES-KOH buffer, pH 7.2.
2.2. Cell preparations
NSC-34 neuronal cells were originally produced by fusion of motor neuron-enriched, embryonic mouse spinal cords with mouse neuroblastoma [13, 14]. They were routinely maintained in 1:1 mixture of DMEM and Ham’s F12 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1%
penicillin-streptomycin. Cultures were incubated at 37 °C in a humidified environment of 5% CO2/95% air.
The medium was often replenished every 2-3 days for removal of non-adhering cells. To slow cell proliferation and enhance their maturation towards a differentiated state, before confluence, cells were grown in 1:1 DMEM plus Ham’s F12 medium supplemented with 1% FBS. NG108-15 mouse neuroblastoma x rat glioma hybrid cells, obtained from the European Collection of Cell Cultures (ECACC-88112302; Wiltshire, UK), were grown in monolayer cultures at a density of 106/ml in plastic disks containing DMEM supplemented with 100 µM hypoxanthine, 1 µM aminopterin, 16 µM thymidine, and 5% (v/v) FBS as the culture medium [15]. Electrophysiological experiments were performed 5 or 6 days after cells had been cultured (60-80% confluence). To observe neurite growth in these cells, a Nikon Eclipse Ti-E inverted microscope (Li Trading Co., Taipei, Taiwan) equipped with a five-megapixel cooled digital camera was
commonly used. The camera was connected to a personal computer controlled by NIS-Element BR3.0 software (Nikon; Kanagawa, Japan).
2.3. RNA isolation and reverse transcriptase-polymerase chain reaction (RT-PCR)
To detect the expression of KV3.1 mRNA in NSC-34 cells, a semi-quantitative RT-PCR assay was performed. Total RNA samples were extracted from NSC-34 cells with TRIzol reagent (Invitrogen) and reverse-transcribed into complementary DNA using Superscript II reverse-transciptase (Invitrogen). The sequences of oligonucleotide primers used for KV3.1 (NM_008421) were 5’-CGTGCCGACGAGTTCTTCT-3’ and 5’-GGTCATCTCCAGCTCGTCCT-3’. Amplication of KV3.1 was made using PCR SuperMix from Invitrogen under the following conditions: 35 cycles composed of 30 sec denaturation at 95 °C, 30 sec primer annealing at 62 °C, 1 min extension at 72 °C, and followed by 72 °C for the final extension for 2 min. PCR products were analyzed on 1.5% (v/v) agarose gel containing ethidium bromide and then visualized under ultraviolet light. Optical densities of DNA bands were scanned and quantified by AlphaImager 2200 (ProteinSimple; Santa Clara, CA, USA).
2.4 Electrophysiological measurements
NG108-15 or NSC-34 cells used for electrophysiological experiments were dissociated and an aliquot of cell suspension was transferred to a recording chamber mounted on the stage of an inverted fluorescent microscope (CKX-41; Olympus, Tokyo, Japan). Cells were bathed at room temperature (20-25 °C) in normal Tyrode’s solution containing 1.8 mM CaCl2. Patch pipettes were made from Kimax-51 glass capillaries (Kimble; Vineland, NJ, USA) using a PP-830 electrode puller (Narishige, Tokyo, Japan) or a P-97 Flaming/Brown micropipette puller (Sutter; Novato, CA, USA), and their tips were fire-polished with an MF-83 microforge (Narishige). The pipettes used had a resistance of 3-5 MΩ when immersed in different solutions as described above. Patch-clamp recordings were made in cell-attached or whole-cell configurations using an RK-400 (Bio-Logic, Claix, France) or an Axopatch 200B patch-clamp amplifier (Molecular Devices; Sunnyvale, CA, USA) [15, 16].
2.5. Data analyses
The inhibitory effect of Flu on IK(DR) in NSC-34 cells can be explained by a state-dependent blocker that binds to the open state of the channel according to a minimal kinetic scheme [16, 17]:
where α and β are the voltage-dependent rate constants for the opening and closing of the delayed-rectifier K+ (KDR) channel; kOB and kBO, those for blocking and unblocking by Flu (i.e., the on- and off-rate constants for Flu) and [B] is the blocker (i.e., Flu) concentration. C, O and O•B represent the closed, open, and open-blocked states, respectively.
Specifically, the blocking and unblocking rate constants, kOB and kBO, were determined from the inactivation time constants (τinact) for IK(DR) evoked by the depolarizing pulses. Those values were estimated using the relation: 1/τinact = kOB × [B] + kBO, where kOB and kBO, respectively, result from the slope and the y-axis intercept at [B] = 0 of the linear regression interpolating the reciprocal time constants (i.e., 1/τinact) versus different Flu concentrations.
The steady-state inactivation curve of IK(DR) with or without addition of Flu was plotted against the conditioning pulses and fitted to the Boltzmann equation:
) / ) exp((
1 1/2
max
k V V I I
−
= +
where V is the conditioning potential in mV, V1/2 is the membrane potential for half-maximal inactivation, and k is the slope factor of inactivation curve for IK(DR).
The relationships between the membrane potentials and the IK(M) conductance obtained before and after the addition of Flu (3 µM) were fitted with a Boltzmann function of the following form:
− − +
=
RT qF V G V
G
) exp (
1
1
2 / max 1
where Gmax is the maximal conductance of IK(M), V1/2 is the voltage at which there is half-maximal activation, q is the apparent gating charge, F is Faraday’s constant, R is the universal gas constant, and T is the absolute temperature. Curve-fitting to data sets presented here was commonly performed using either Microsoft Excel (Redmond, WA, USA) or Origin 8.0 (OriginLab, Northampton, MA, USA).
Values are provided as means±SEM with samples sizes (n) indicating the number of cells from which the data were taken. The paired or unpaired Student’s t-test and one-way analysis of variance (ANOVA) with the least significant different method for multiple comparisons were used for the statistical evaluations of
differences among means. Non-parametric Kruskal-Wallis test was then used if the assumption of normality underlying ANOVA was violated. Statistical significance was determined at a P-value of <0.05.
3. Results
3.1. Effect of Flu on delayed rectifier K+ current (IK(DR)) in motor neuronal NSC-34 cells
In this study, the whole-cell configuration of the patch-clamp technique was used to evaluate effects of Flu on ion current in NSC-34 cells. To record K+ outward currents, cells were bathed in Ca2+-free Tyrode's solution which contained tetrodotoxin (1 µM) and CdCl2 (0.5 mM). When the cell was held at -50 mV, various voltage pulses ranging from -100 to +40 mV in 10-mV increments were applied with a duration of 1 sec, a family of K+ currents was elicited (Fig. 1). These outward currents which were subject to block by tetraethylammonium chloride or 4-aminopyridine, but not by iberiotoxin or apamin, was thus identified as IK(DR) [16, 17]. Iberiotoxin and apamin are blockers of the large- and small-conductance Ca2+-activated K+ channels, respectively. When cells were exposed to Flu (3 µM), the IK(DR) amplitude was reduced together with increased current inactivation. For example, when cells were depolarized from -50 to +40 mV, Flu at a concentration of 3 µM significantly decreased the current amplitude by 30±2% from 581±32 pA to 404±26 pA (n=11). After washout of drug, current amplitude was partially returned to the control. The I-V relationships of IK(DR) with or without addition of Flu are illustrated (Fig. 1B). It was also noted that the current amplitudes at the voltages between -50 and +10 mV were increased in the presence of Flu. Fig. 1C depicts an I-V relationship of Flu-sensitive current which could not result from the contamination with either Na+ or Ca2+ currents, because bathing solution in this study contained tetrodotoxin and CdCl2.
3.2 Kinetic studies of Flu-induced block of IK(DR)
Because IK(DR) observed in the presence of Flu tends to exhibit a pronounced peak followed by an exponential decay to a steady-state level, it is necessary to evaluate the kinetics of Flu-induced block of IK(DR)
in NSC-34 cells. To provide more evidence for Flu-induced blocking of IK(DR), the time constants for IK(DR)
inactivation in NSC-34 cells were further analyzed. The time courses of current inactivation in the absence and presence of different Flu concentrations were fitted by a single-exponential function. The concentration dependence of IK(DR) elicited by sustained depolarizations with or without addition of Flu is illustrated in Fig.
2. The results showed that the effects of this compound were concentration-dependent increase in the rate of current inactivation accompanied by a decrease in the residual, steady-state current. For example, when cells were depolarized from -50 to +50 mV with a duration of 10 sec, the inactivation time constants of IK(DR)
during exposure to 1 and 3 µM Flu were fitted by a single exponential with the values of 1687±13 msec (n=8)
and 1509±12 msec (n=9), respectively.
Based on the first-order blocking scheme described in Materials and Methods, the relationship between 1/τinact and [B] became linear with a correlation coefficient of 0.96 (Fig 2C). The blocking and unblocking rate constants measured from eight to eleven different cells were calculated to be 0.0564 sec-1µM-1 and 0.504 sec-1, respectively. Thereafter, based on these values, a value of 8.9 µM for the dissociation constant (KD = kBO/kOB) could be calculated.
Previous studies have demonstrated that Flu could activate IK(M) [9]. We also examined whether linopirdine, a blocker of IK(M), had any effects on Flu-induced perturbations of IK(DR) amplitude. As shown Fig. 2D, linopirdine at a concentration of 10 µM had little or no effect on the inactivation time constant of IK(DR) elicited by membrane depolarization. Therefore, Flu-induced elevation of IK(DR) inactivation rate in NSC-34 cells did not result from the activation of IK(M).
3.3. Lack of effect of N-methyl-D-aspartate (NMDA) or gabazine on Flu-induced changes in IK(DR)
Several reports showed that Flu could interact with either glutamate NMDA or GABA receptors, thereby influencing the activity of ion channels in central neurons [5-7, 18]. We further evaluated whether the challenging of cells with NMDA, an NMDA agonist, or gabazine, a blocker of GABAA receptor, can exert any effects on Flu-induced changes in IK(DR) in these cells. The experiments were conducted with a 10-sec depolarizing pulse from a holding potential of -50 mV. Current amplitude at the end of each depolarizing pulse was measured and the inactivation time constant for IK(DR) was analyzed. As shown in Fig. 3, subsequent application of NMDA (30 µM) or gabazine (10 µM) had minimal effects on both current amplitude and inactivation elicited by long-lasting membrane depolarization. The observed effect of Flu on IK(DR) inactivation is thus unlinked to the binding to NMDA or GABA receptors and most likely due to the drug acting on the channel itself.
3.4. Steady-state inactivation of IK(DR) obtained with or without addition of Flu
To characterize the inhibitory effect of Flu on IK(DR) in NSC-34 cells, we next investigated the voltage-dependence of its effect on IK(DR) using a double-pulse protocol. Figure 4 shows the steady-state inactivation curve of IK(DR) in the absence and presence of Flu (3 µM). In these experiments, a 10-sec conditioning pulse to different potentials preceded the test pulse to +50 mV from a holding potential of -50
derived and constructed with a Boltzmann function, as described in Materials and Methods. In controls, voltage for half-maximal inactivation (V1/2) and corresponding slope factor (k) were -9.5±0.4 mV and 7.4±0.3 mV (n=9), respectively, while in the presence of Flu (3 µM), the values of V1/2 and k were -15.9±0.6 mV and 7.2±0.3 mV (n=10), respectively. Therefore, addition of Flu slightly but significantly shifted the midpoint of the inactivation curve toward hyperpolarizing voltage by approximately 6.4 mV; however, no significant change in the slope factor was detected during exposure to Flu.
3.5. Flu-induced increase in cumulative inhibition of IK(DR) inactivation
In another set of experiments, we sought to determine whether Flu can alter the time course of IK(DR) in response to repetitive stimuli in NSC-34 cells. Under control condition, a single 10-sec depolarizing step to +40 mV from a holding potential of -60 mV produced an exponential decline with a time constant of 4.09±0.08 sec (n=8). However, the time constant for 10-sec repetitive pulses to +40 mV, each of which lasted 20 msec with 10-msec interval at -60 mV between the depolarizing pulses, was significantly reduced to 3.51±0.07 sec (n=7). Additionally, as depicted in Fig. 5, the results shows a progressive increase in the decline of IK(DR) in response to rapid depolarizing stimuli. When the cells were exposed to Flu, the value of time constant obtained during this train of short repetitive pulses was further reduced. Addition of 1 and 3 µM Flu decreased the time constants to 2.54±0.08 sec (n=7) and 1.81±0.07 sec (n=6), respectively. However, subsequent application of linopirdine (10 µM) did not alter the inactivation time constant for IK(DR) in response to such repetitive stimuli (data not shown). Therefore, it needs to be noted that an excessive accumulative inactivation of IK(DR) in the presence of Flu can be observed and not directly relevant to its stimulation of IK(M).
3.6. Stimulatory effect on IK(M) in NSC-34 cells
Previous studies have demonstrated the presence of IK(M) in NG108-15 neuronal cells [15, 19]. The experimental results shown in Fig. 1 suggest that Flu can increase the IK(DR) amplitude recorded at the voltages between -50 and +10 mV. We next investigated the effect of Flu on IK(M) found in NSC-34 cells. When cells were hyperpolarized from -20 to -50 mV, the IK(M) amplitudes in the presence of Flu were significantly greater than those in the control. Similarly, addition of meclofenamic acid (30 µM), an activator of KCNQ2/Q3 channels [20], elevated the amplitude of IK(M) (data not shown).
Fig. 6C shows the activation curve of IK(M) in the absence and presence of Flu (3 µM). The plot of
relative IK(M) conductance as a function of membrane potential was fitted with a Boltzmann function as described under Materials and Methods. In controls, V1/2=-18.3±0.6 mV, q=2.25±0.08 e (n=8), whereas in the presence of Flu (3 µM), V1/2=-32.6±1.1 mV and q=2.31±0.09 e (n=7). The data showed that the activation curve was shifted along the voltage axis to more negative potentials by approximately 14 mV, as NSC-34 cells were exposed to Flu. In contrast, no significant change in the gating charge was demonstrated during exposure to Flu. These results thus indicate that Flu is capable of stimulating IK(M) in a voltage-dependent fashion in NSC-34 cells.
3.6. Effect of Flu on IK(DR) in NG108-15 neuronal cells
Previous studies in our laboratory have demonstrated the presence of IK(DR) (i.e., KV3.1-encoded current) in NG108-15 neuronal cells [15-17]. In a final set of experiments, to verify whether Flu-induced inhibition of IK(DR) could also be observed in other types of neuronal cells, we examined the effects of this compound on NG108-15 neuronal cells. As shown in Fig. 7, Flu was effective in suppressing IK(DR) enriched in these cells.
Besides that, it was able to raise the inactivation rate of IK(DR) in response to a long-lasting depolarizing pulse.
Similar to the results in NSC-34 cells, linopirdine at a concentration of 10 µM was unable to alter the inactivation time constant of IK(DR) decreased by Flu.
4. Discussion
This study demonstrated that in motor neuronal NSC-34 cells, Flu, which is a centrally acting, non-opioid analgesic, induced a time-, concentration-, and state-dependent decay of IK(DR), although no change in activation kinetics of this current was found. These observations, along with a good description of IK(DR)
inactivation time course at different Flu concentrations with the simulation of minimal binding scheme, led us to propose that Flu may act as a state-dependent blocker for IK(DR). Block by Flu of IK(DR) is potentially important because it may have characteristics that render it significant from a pharmacological viewpoint.
In our study, NSC-34 cell line was found to exhibit the activity of the slowly inactivating delayed rectifier K+ channels as described in NG108-15 neuronal cells [16, 17]. A notable characteristic for the majority of IK(DR) in NSC-34 cells is that it activates rapidly and then inactivates slowly. The activation time constant ranges from 20 to 50 msec at the potentials between +30 and +70 mV; however, it tends to inactivate slowly from several hundreds to several thousands of milliseconds during sustained depolarization. The kinetics of KV3.1-encoded current thus fit well with these properties [16, 17, 21]. It is anticipated that KV3.1-encoded currents are the major molecular component of IK(DR) in NSC-34 cells. However, because neither margatoxin (100 nM) nor agitoxin-2 (100 nM) produce any effects on IK(DR) in NSC-34 cells (data not shown), there is most likely to be lack of functional expression of KV1 subunits in these cells. Margatoxin is a selective inhibitor of KV1.3 channel and agitoxin is a blocker to Shaker K+ channels.
The observed effect of Flu on IK(DR) in NSC-34 cells clearly did not involve the suppression of Ca2+-dependent K+ channels. The recordings of IK(DR) presented herein were commonly conducted in a Ca2+-free Tyrode's solution containing CdCl2 (0.5 mM). Moreover, neither peak amplitude nor inactivation kinetics of IK(DR) in NSC-34 cells remained altered during exposure to iberiotoxin or apamin. Iberiotoxin and apamin are known to block the large- and small-conductance Ca2+-activated K+ channels, respectively.
However, the block of IK(DR) by Flu could be responsible for the widening of neuronal action potentials [16, 21].
In this study, NMDA, an NMDA receptor agonist, did not produce any effects on Flu-induced reduction of IK(DR); therefore, the inhibitory effect of Flu on IK(DR) described here would not be linked to an interaction with NMDA receptors. Additionally, gabazine (10 µM), a blocker of GABAA receptor, also did not exert any effect on Flu-induced reduction of IK(DR) inactivation and amplitude. Therefore, Flu-induced
perturbations in IK(DR) kinetics is not because of the ability to bind to GABAA receptors.
The KD value of Flu required for the increase of IK(DR) inactivation rate was 8.9 µM. Linopirdine did not reverse Flu-induced increase of IK(DR) inactivation rate. After a single oral application of 200 mg Flu or the therapeutic daily dose of 600 mg Flu, the peak plasma concentration of 2.4 µg/ml (6 µM) can be reached [1, 9, 22]. It is possible that the Flu concentration used to alter the kinetics of IK(DR) is compatible with clinically relevant concentrations.
We also demonstrated the presence of IK(M) in NSC-34 cells. Addition of Flu was noted to increase IK(M)
amplitude and shift the activation curve of this curve to more negative potentials, although no change in the gating charge was demonstrated. Because of lack of Flu on the gating charge in IK(M), the voltage-sensor regions of neuronal KCNQ channels might not be altered by this compound. Subsequent application of linopirdine can reverse Flu-stimulated IK(M). Besides that, the effects of Flu and meclofenamic acid on the IK(M) amplitude in NSC-34 cells were not additive (data not shown), suggesting that the two compounds exert their major effects on the same component. However, the ability of Flu to suppress IK(DR) amplitude and to increase the inactivation time course of this current in neurons is necessarily noted with caution in relation to its use as an activator of neuronal KCNQ channels.
The KV3.1-encoded channels were enriched in many central neurons including hippocampal pyramidal neurons, auditory neurons and Purkinje cells [21]. The activity of these channels is recognized to participate in electrical behaviors of fast-spiking neurons [21, 23]. Addition of Flu reduced the peak amplitude of IK(DR)
as well as increased the inactivation time course of this current recorded from NSC-34 cells. The present observations would clearly initiate further studies to understand the Flu effects on electrical activity of motor neurons [4, 24, 25]. Whether Flu used in treating patients with Creutzfeld-Jakob disease [1-3, 26] relates to an inhibition of IK(DR) needs further evaluations.
In summary, both stimulation of IK(M) and inhibition of IK(DR) caused by Flu may synergistically act to diminish the firing activity of motor neurons if similar results occur in vivo. Our results are novel but are based on two different types of neuronal cells and require replication in central neurons from different regions.
Nonetheless, in addition to its stimulatory effect on IK(M), the slowly inactivating IK(DR) presented herein may be a relevant target for the action of Flu, if these types of K+ currents perturbed by this agent are present in
Acknowledgments
This work was, in part, supported by grants from the National Science Council (NSC-98-2320-B-006-MY3) and the Aim for the Top University Project, National Cheng Kung University, Taiwan. We gratefully acknowledge Dr. Ya-Jean Wang and Hsien-Chin Huang for their assistances in participating in early experiments.
Conflicts of interest
The authors declare no conflicts of interest that are directly relevant to this study.
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Figure Legends
Figure 1. Effects of Flu on IK(DR) in NSC-34 neuronal cells. Cells were bathed in Ca2+-free Tyrode's solution containing tetrodotoxin (1 µM) and CdCl2 (0.5 mM). (A) Superimposed current traces in the absence (upper) and presence (lower) of 3 µM Flu. The cell examined was held at -50 mV and various potentials ranging from -100 to +40 mV in 10-mV increments as shown in the uppermost part of (A) were applied. (B) Averaged I-V relations for K+ currents measured at the end of each voltage pulse in the absence (■) and presence (□) of 3 µM Flu (mean±SEM; n=9-13 for each point). (C) The I-V relationship of Flu-sensitive current (i.e., the difference of averaged K+ currents between the absence and presence of 3 µM Flu).
Figure 2. Evaluation of the kinetics of Flu-induced block of IK(DR) in NSC-34 neuronal cells. In (A), the time courses of current inactivation obtained in the absence (a) and presence of 1 µM (b) and 3 µM (c) Flu
were well fitted by a single exponential shown in (B). Inset in (A) indicates the voltage protocol used. In (C), the kinetics of Flu-induced block of IK(DR) in NSC-34 cells was evaluated. The reciprocal of inactivation time constant of IK(DR) (1/τinact) obtained by exponential fit to the trajectory of IK(DR) decay was plotted against the Flu concentration. Data points were fitted by a linear regression shown in blue line, indicating that Flu-induced blocking occurs with a molecularity of 1. Blocking (kOB) and unblocking (kBO) rate constants, given by the slope and the y-axis intercept of the interpolated line, were 0.0564 sec-1µM-1 and 0.504 sec-1, respectively. (D) Summary of the data showing the effect of Flu and linopirdine (Lino; 10 µM) on the inactivation time constants (τinact) of IK(DR) elicited by a 10-sec depolarizing pulse from -50 to +50 mV (mean±SEM; n=8-10 for each point). In the experiments with Flu plus linopirdine, linopiridine (10 µM) was applied after the addition of Flu (3 µM). *Significantly different from control.
Figure 3. Lack of effect of NMDA or gabazine on Flu-induced effect on IK(DR) in NSC-34 cells. In these experiments, each cell was depolarized from -50 to +50 mV with a duration of 10 sec. Current amplitude at the end of depolarizing pulse was measured and current inactivation time course was fitted by a single exponential. Bar graphs in (A) and (B) show the effects of Flu (3 µM) , Flu (3 µM) plus NMDA (30 µM), and Flu (3 µM) plus gabazine (10 mM) on the peak amplitude (A) and inactivation time constant (B) of IK(DR), respectively. In the experiments with Flu plus NMDA or gabazine, NMDA (30 µM) or gabazine (10 µM) was applied after the addition of Flu (3 µM). Each bar represents the mean±SEM (n=8-12). *Significantly different from controls. Notably, subsequent application of NMDA or gabazine did not alter the amplitude and inactivation time course of IK(DR).
Figure 4. Effect of Flu on steady-state inactivation of IK(DR) in NSC-34 cells. (A) Superimposed current traces obtained in the absence (upper) and presence (lower) of 3 µM Flu. The conditioning voltage pulses with a duration of 10 sec to various membrane potentials. The voltage protocol used is shown in the uppermost part. (B) Steady-state inactivation of IK(DR) obtained in the absence (■) and presence (□) of 3 µM Flu (mean±SEM; n=8-11 for each point).
presence (lower) of 3 µM Flu recorded from motor neuronal NSC-34 cells. Currents were obtained during a 20-sec repetitive depolarizations to +40 mV from a holding potential of -60 mV. The uppermost part indicates the voltage protocol used. Note that in addition to the inhibition of IK(DR) amplitude, Flu increases the rate of excessive accumulative inactivation of IK(DR) elicited by repetitive stimuli.
Figure 6. Stimulatory effect of Flu on M-type K+ current (IK(M)) in NSC-34 cells. In these experiments, cells were bathed in Ca2+-free Tyrode's solution and the pipette was filled with a K+-containing solution. (A) Current traces obtained in the absence (a) and presence of 1 and 3 µM Flu. The inset indicates the voltage protocol used. (B) Summary of the data showing effects of Flu and linopirdine on IK(M) measured at -50 mV (left) and -20 mV (right) in NSC-34 cells (mean±SEM; n=10-13 for each point). Flu: flupirtine; Lino:
linopirdine. *Significantly different from controls. **Significantly different from Flu (3 µM) alone group.
(C) Voltage dependence of IK(M) activation in the absence (■) and presence (□) of 3 µM Flu (mean±SEM;
n=7-10 for each point). The smooth dashed lines represent the best fits to the Boltzmann equation described in Materials and Methods. Notably, addition of Flu can shift the activation curve of IK(M) to the hyperpolarizing voltage with no change in the gating charge of this current.
Figure 7. Inhibitory effect of Flu on IK(DR) in NG108-15 neuronal cells. Cells were bathed in Ca2+-free Tyrode's solution and IK(DR) was elicited by the depolarizing pulse from -50 to +50 mV with a duration of 10 sec. a: control; b: 1 µM Flu; and c: 3 µM Flu. The inset indicates the voltage protocol used. Notably, addition of Flu increases the IK(DR) inactivation rate along with the reduced current amplitude.
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