國 立 交 通 大 學
環 境 工 程 研 究 所
碩 士 論 文
利 用 密 閉 式 藻 類 毒 性 試 驗 評 估
鄰 苯 二 甲 酸 酯 類 之 毒 性
(A novel algal toxicity testing technique for assessing the
toxicity of benzenedicarboxylate)
研 究 生 :柯 嘉 玟
指 導 老 師:陳 重 元 教授
利 用 密 閉 式 藻 類 毒 性 試 驗 評 估 鄰 苯 二 甲 酸 酯 類 之 毒 性
A novel algal toxicity testing technique for assessing the toxicity of benzenedicarboxylate
學生:柯嘉玟 Student:Chia-wen Ko 指導教授:陳重元 Adviser:Dr. Chung-Yuan Chen
國立交通大學 環境工程研究所
碩士論文
A Thesis
Submitted to Institute of Environmental Engineering College of Engineering
Nation Chiao Tung University In Partial Fulfillment of the Requirements
For the Degree of Master of Science In
Environmental Engineering July 2006
Hsinchu, Taiwan, Republic of China
利用密閉式藻類毒性試驗評估鄰苯二甲酸酯類之毒性
學生:柯嘉玟 指導教授:陳重元 老師 國立交通大學環境工程研究所摘要
摘要
摘要
摘要
本研究結果發現 14 個鄰苯二甲酸酯類及 4 個對苯、2 個間苯二甲酸酯 類在 BOD 瓶藻類毒性試驗之下皆具有急毒性,毒性隨碳鍊增長而變強, 且三個反應參數(△DO、Final yield 及 Growth rate)下是以△DO 敏感度最 高﹔與其他物種比較的部分,發現除了低分子之化合物敏感度較低之外, 敏感度都比其他物種的高,若是與其他藻種比較,則敏感度上的表現是差 不多的﹔替代物種部分,月芽藻與 Fathead minnow 及 Water flea 迴歸結果 R2在 0.89〜0.93,因此對於此類化合物可以利用藻類來預估較高營養階層 的魚類與水蚤類。QSAR 的結果發現若去除接有較強的氫離子游離能力的 4 個胺基及硝基之化合物及另外兩個 outliers 點(Diphenyl Phthalate、Butyl (2-ethylhexyl) Phthalate)後,迴歸毒性與 log P 的結果得到不錯的 R2值 0.88 〜0.92,若單純討論鄰苯二甲酸酯類會得到更高的 R2值 0.93〜0.95 之間,進而使用 log P*MW 當作參數得到更高的 R2值 0.95〜0.96;最後利用基線
毒性預測此類化合物,發現除了 outliers 之外,殘差值都界於±1 個 log 單 位間,因此判斷其餘化合物是屬於非極性麻醉毒性機制的。
phthalate esters
Student:Chia-wen Ko Adviser:Chung-Yuan Chen
Institute of Environmental Engineering National Chiao Tung University
ABSTRACT
This paper presents the toxicity data of benzenedicarboxylate(14 phthalate esters、4 terephthalate esters and 2 isophthalate esters) on Psudokirchneriella
subcapitata (green algae) assessed by a new algal toxicity testing technique conducted under air-tight environment. Based no DO production, median effect concentration (EC50) varies from 0.037 mg/L (Dihexyl phthalate, DHP) to 344
mg/L(Bis(Methoxyethyl) phthalate, BMOEP). The endpoint of algal DO production reveals more sensitive that from final yield and growth rate. Compared to literature data, Psudokirchneriella subcapitata are apparently more sensitive to the other organisms such as Fathead minnow、Daphnia
magan or T. pyroformis besides the low molecular phthalate(Dimethyl
phthalate and Diethyl phthalate). Furthermore, good correlations between toxic effects observed from Psudokirchneriella subcapitata and other aquatic
organisms were found. Hence, algal toxicity test can be considered as a
surrogate test for estimating the toxicity of phthalate esters to Fathead minnow and Daphnia magna.
Quantitative Structure-Activity relationship (QSAR) was established based on chemical hydrophobicity , 1-octanol/water partition coefficient (log P) with R2 equal to 0.88〜0.92. If the parameter of log P* Molecular weight is used, we can get a greater R2 0.95〜0.96. The purpose we used this parameters was not only to get a great R2, but to describe the physiological aspects relative to the mode of action of the chemicals assayed.
Finally, we used the equation of baseline toxicity built by our laboratory, only six compounds had more than 1 residual. According to this result, we determined the mode of action of other 14 compounds were non-polar toxicity.