II
骨頭形成蛋白與鈣磷骨水泥混合物對於骨頭形成影響的研究：
模擬脊椎成形術的動物實驗
The effect of rhBMP and calcium phosphate cement (CPC) composites on the new bone
formation: An animal study based on vertebroplasty simulation
III
植入位置示意圖
其分組如下：
IV
圖3-1 Control 1(empty) 4 weeks
V
圖3-3 Control 2(CPC) 4 weeks
VI
圖3-5 CPC + 10mg BMP 4 weeks
VII
圖3-7 CPC + 30mg BMP 4 weeks
VIII
上面的圖為，我們將植入後的脊椎取出所拍的照片。紅色圈圈部分為我們所植
入的地方，由圖可以看出骨頭上方有一層纖維組織包覆住，這是身體裡面的細胞所
啟動的自動防衛功能，會將發炎的地方以一層纖維組織包住
。為了更進一步分析裡面的結構，我們做了組織切片。採用的是 H&E strain 來染
色並觀察其結果，以下的圖為 H&E strain 試片在 OM 15 倍放大倍率下的照片。
圖 3-9 2 mm hole after 4 weeks 圖 3-10 2 mm hole after 8 weeks
IX
圖 3-13 with 10mg BMP after 4 weeks 圖 3-14 with 10mg BMP after 8 weeks
圖 3-15 with 30mg BMP after 4 weeks 圖 3-16 with 30mg BMP after 8 weeks
X T3 cpc 10mg BMP 30mg BMP 0 20 40 60 80 100 120 T3 cpc + BMP
WITH T3 solution in HANK solution 1 day 1 day 1 day 4 weeks 4 weeks 4 weeks 8 weeks 8 weeks 8 weeks C O M P R E S S IV E S T R E N G T H ( M P a ) setting solution 圖 3-17 cpc 與不同 BMP 添加量
此外我們可以在 CPC + BMP 的圖中看出，新組織明顯長入。由圖 3-17 可看出，
添加 BMP 會讓整體強度下降，但還在可以接受的範圍內。
四 結論
BMP的添加雖然讓我們實驗室自製的cpc整體強度下降，但其下降範圍還在可以
接受的程度以內，且因為Liquid/powder ratio變大的關係，讓整體空隙度變大，
雖然吸收降解的速度會變快很多，但由於我們加入了適量的骨頭形成蛋白（ＢＭＰ）
加速了新組織的形成，整體上，此種作法是極為有利的。
五 參考文獻
Bai BB, Kummer FJ, Spivak J. Augmentation of anterior vertebral body screw fixation by an injectable,
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Constantz BR, Ison IC, Fulmer MT, et al. Skeletal repair by in situ formation of the mineral phase of bone.
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