飲食中給予
飲食中給予 glutamine補充對於敗血症小鼠體內 glutamine 補充對於敗血症小鼠體內
Th1/Th2
Th1/Th2平衡的改變及其器官中 平衡的改變及其器官中 IL- IL -6 6表現之影響 表現之影響
實驗目的實驗目的
: :過去的研究顯示，敗血症時體內
過去的研究顯示，敗血症時體內Th1/Th2 Th1/Th2的平衡會改變，而偏向
的平衡會改變，而偏向Th2 Th2反應會使病人
反應會使病人 更易造成器官衰竭。發生敗血症時給予更易造成器官衰竭。發生敗血症時給予
Glutamine(Gln)添加有助於全身性免疫系統的調節，然 Glutamine(Gln)
添加有助於全身性免疫系統的調節，然 而對於局部器官中免疫力的改變及而對於局部器官中免疫力的改變及
T淋巴球內的細胞激素的調節目前並無相關的研究，因此本 T
淋巴球內的細胞激素的調節目前並無相關的研究，因此本 實驗期望能透過給予小鼠飲食中實驗期望能透過給予小鼠飲食中
Gln Gln的添加，來觀察敗血症後器官組織中細胞激素
的添加，來觀察敗血症後器官組織中細胞激素IL- IL -6 6的濃度
的濃度 變化，及淋巴球中變化，及淋巴球中
Th1/Th2 Th1/Th2
的平衡改變的情形。的平衡改變的情形。實驗方法實驗方法
: :
Normal control (NC) group:
Normal control (NC) group:餵食
餵食chow chow 飲食
飲食Control group:
Control group:餵食
餵食semi purified semi purified 飲食
飲食Gln group:
Gln group:部分
部分casein casein由
由Gln取代的飲食 Gln
取代的飲食(week) 0 0
適應期適應期1 1 4 4
利用利用
cecal cecal ligation ligation and puncture (CLP) and puncture (CLP)
引致敗血症引致敗血症
(sepsis) (sepsis)
Sepsis
Sepsis 後
後0,6,12 0,6,12及
及24小時犧牲老鼠 24
小時犧牲老鼠雄性雄性ICR miceICR mice
實驗結果 實驗結果 : :
Component Component (g/kg)
(g/kg) ControlControl GlutamineGlutamine Soybean oil
Soybean oil 100100 100100 Casein
Casein 200200 150150
Glutamine
Glutamine 00 41.741.7
Salt mixture
Salt mixture 3535 3535
Vitamin Vitamin mixture
mixture 1010 1010
Methy Methy cellulose
cellulose 3131 3131
Choline Choline chloride
chloride 11 11
Methionine
Methionine 33 33
Corn starch
Corn starch 620620 628.3628.3 Table 1.
Table 1. Composition of the experimental Composition of the experimental diets
diets
結論結論
: :
給予給予Gln補充可降低老鼠敗血症時肝外器官如 Gln
補充可降低老鼠敗血症時肝外器官如:肺、腎及小腸組織中促發炎細胞激素 :
肺、腎及小腸組織中促發炎細胞激素IL- IL -6 6的濃度，但肝中
的濃度，但肝中IL
IL- -6 6
的濃度則可維持或可有助於促進急性期反應。對於淋巴球中的濃度則可維持或可有助於促進急性期反應。對於淋巴球中Th1 Th1
細胞激素細胞激素IFN IFN- -
γ的濃度則有促進而γ的濃度則有促進而Th2 Th2
細胞激素細胞激素
IL- IL -4 4則有抑制的作用，此結果將會促使敗血症時體內偏向
則有抑制的作用，此結果將會促使敗血症時體內偏向Th1 Th1的免疫反應。
的免疫反應。LungLung KidneyKidney IntestinesIntestines Pg/mg tissue
Pg/mg tissue NC group
NC group 1.331.33±±0.240.24 12.112.1±±0.90.9 1.051.05±±0.730.73 6h
6h
Control group Control group Gln group
Gln group 1.991.99±±0.560.56
1.701.70±±0.560.56 12.3512.35±±0.970.97 11.56
11.56±±2.332.33 1.021.02±±0.250.25 1.571.57±±1.681.68 12h12h
Control group Control group Gln group Gln group
28.3 28.3±±7.187.18**‡‡＃＃
11.9 11.9±±1.71.7‡‡＃＃
31.6 31.6±±1.081.08*‡*‡＃＃
28.9 28.9±±3.043.04‡＃‡＃
27.1 27.1±±0.860.86*‡*‡＃＃
15.1 15.1±±2.882.88‡＃‡＃ 24h24h
Control group Control group Gln group Gln group
13.813.8±±3.183.18*‡*‡ 5.54 5.54±±2.542.54
26.96 26.96±±0.750.75**‡‡
18 18±±0.910.91‡‡
3.53.5±±0.660.66‡‡ 4.03 4.03±±0.820.82‡‡ 0h
0h
Control group Control group Gln group Gln group
15.1 15.1±±2.472.47*‡*‡
1.231.23±±0.030.03
20.2 20.2±±0.610.61**‡‡
13.9±13.9±1.441.44
3.58 3.58±±0.80.8‡‡ 2.082.08±±1.321.32 Table 2. The concentration of IL
Table 2. The concentration of IL--6 in lung, kidney, and 6 in lung, kidney, and intestines tissue homogenate during sepsis
intestines tissue homogenate during sepsis
*: significantly different from correspondent group in the same time point
‡: significantly different from normal control group and time 0h in the same group
#: significantly different from the same group at various time points
0 5 10 15 20 25
NC 0 6 12 24
Control Gln NC
ILIL--6 (pg/mg tissue)6 (pg/mg tissue)
Time after CLP (h) Time after CLP (h)
Figure 1. The concentration of IL-6 in liver homogenate during sepsis
* : significantly different from correspondent group in the same time point; †:
significantly different from NC group and time 0h in the same group
‡*‡
‡
*‡
‡
*
0 20 40 60 80 100
NC 0 6 12 24
Control Gln NC
Distribution (%)Distribution (%)
Time after CLP (h) Time after CLP (h) Figure 2.Distributions of Intra-Lymphocytes IFN-γ
at the time indicated after CLP-induced mice sepsis;
‡＃
*‡＃
‡*‡
‡
*‡
0 0.5 1 1.5 2 2.5 3
NC 0 6 12 24
Control Gln NC
Distribution (%)Distribution (%)
Time after CLP (h) Time after CLP (h)
Figure 3.Distributions of Intra-Lymphocytes IL-4 at the time indicated after CLP-induced mice sepsis
‡
‡ *
*＃‡
